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Effect of boron on microspore embryogenesis and direct embryo to plant conversion in Brassica napus (L.)

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Abstract

The NLN-medium has been successfully used, since 1982, for microspore culture in Brassica napus and other Brassica species. Changes to the media composition were restricted to carbohydrate and nitrogen sources and growth regulators while micro-nutrients have not been optimized. The NLN-medium contains boron at a concentration of 162 µM. Boron is required for diverse physiological and metabolic processes in the cell. This study investigated the effect of seven- and 13-fold increased boron concentration on the induction of embryos in microspore cultures of four genotypes of B. napus. A significant improvement of microspore embryogenesis was achieved by both enhanced boron concentrations in the NLN medium. No effect on the regeneration of embryo to plant conversion was observed.

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Acknowledgements

PM and MCMI thank the Erasmus-Mundus Action II (Experts-Sustain) program of the EU for research fellowships.

Author contributions

CM and PM planned the experiment. PM, ASK and MCI performed the experiments. CM and PM analysed the data and all authors discussed the results. CM wrote the manuscript draft which was further improved by PM and MCI. All authors agreed to the final version of the manuscript.

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Authors and Affiliations

  1. Department of Crop Sciences, University of Göttingen, Von-Siebold-Str. 8, 37075, Göttingen, Germany

    Pitchayapa Mahasuk, Annika Stina Kullik & Christian Möllers

  2. Plant and Environmental Sciences, National Institute of Fundamental Studies, Hantana Road, Kandy, 20000, Sri Lanka

    Mohammed Cassim Iqbal

Authors
  1. Pitchayapa Mahasuk
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  2. Annika Stina Kullik
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  3. Mohammed Cassim Iqbal
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  4. Christian Möllers
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Corresponding author

Correspondence to Christian Möllers.

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The authors declare that they have no conflict of interest.

Additional information

Communicated by Alison M.R. Ferrie.

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Mahasuk, P., Kullik, A.S., Iqbal, M.C. et al. Effect of boron on microspore embryogenesis and direct embryo to plant conversion in Brassica napus (L.). Plant Cell Tiss Organ Cult 130, 443–447 (2017). https://doi.org/10.1007/s11240-017-1232-5

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  • DOI: https://doi.org/10.1007/s11240-017-1232-5

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