Abstract
Arabidopsis plant that is doubly mutant for the de novo methylases DRM1 and DRM2 (drm1/drm2) produce larger and heavier callus than wild type (WT) plants when cultivated on callus induce medium (CIM) for a given period of time. This trend is observed both in seedlings and explants, which indicates that both DRM1 and DRM2 are involved in callus formation. Furthermore, the gene expression analysis revealed that the BBM expression was continuously increased with CIM cultivation, and the expression level was significantly higher in the drm1/drm2 mutants than WT. And the anti-stress response genes have altered in the drm1/drm2 mutants compared with WT, These data suggest that DNA methylation and corresponding genes play an important role in callus formation .
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This paper was supported by Natural Science Foundation of China (Grant No. 31370214).
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Fangwei Jiang and Xiaofeng Xu have contributed equally to this work.
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Jiang, F., Xu, X., Liu, H. et al. DRM1 and DRM2 are involved in Arabidopsis callus formation. Plant Cell Tiss Organ Cult 123, 221–228 (2015). https://doi.org/10.1007/s11240-015-0812-5
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DOI: https://doi.org/10.1007/s11240-015-0812-5