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In vitro propagation and camptothecin production in Nothapodytes nimmoniana

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Abstract

Nothapodytes nimmoniana is an endemic endangered tree and convenient source for large scale isolation of anticancer alkaloid camptothecin. Due to indiscriminate collection of its wood chips for camptothecin, population of the species is under heavy exploitation pressure in India. Plant tissue culture techniques offer alternative strategies for clonal propagation and camptothecin production to meet demand of the alkaloid along with conservation of the species. Several strategies are employed to enhance in vitro response through seed germination, culture medium optimisation for camptothecin production and clonal propagation. Cryopreservation of embryonic axes is an approach employed in short term preservation of the species. Scale up for the production of camptothecin from N. nimmoniana in bioreactor is a future possibility to meet pharmaceutical market demand and mitigate exploitation of natural population. In this article, we reviewed progress made so far on clonal propagation and camptothecin production in N. nimmoniana. We assessed the challenges and status of camptothecin production in N. nimmoniana by application of in vitro techniques.

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Abbreviations

MS:

Murashige and Skoog medium

MMS:

Modified Murashige and Skoog medium

LS:

Linsmaeir and Skoog

B5:

Gamborg’s B5 medium

WPM:

Woody plants medium

PGR:

Plant growth regulators

TDZ:

Thidiazuron

BAP:

Benzylaminopurine

Kin:

Kinetin

2,4-D:

2,4-Dichlorophenoxyacetic acid

2,4,5-T:

2,4,5-Trichlorophenoxyacetic acid

IBA:

Indole 3-butyric acid

IAA:

Indole acetic acid

NAA:

Naphthalene acetic acid

2ip:

2-Isopentenyladenine

GA3 :

Gibberellic acid

ISSR:

Inter-simple sequence repeat interval

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Acknowledgments

This work is carried out under financial support to Tasiu Isah from the Department of Biotechnology, Government of India (New Delhi) and Third World Academy of Science (Strada Costiera, Trieste, Italy) under a DBT-TWAS Postgraduate Research Fellowship. We are highly grateful to Hamdard University (New Delhi) for providing research facilities. We further thank anonymous reviewers for their constructive comments on the manuscript.

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  1. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi, 110062, India

    Tasiu Isah & Abdul Mujib

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  1. Tasiu Isah
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  2. Abdul Mujib
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Correspondence to Tasiu Isah.

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Isah, T., Mujib, A. In vitro propagation and camptothecin production in Nothapodytes nimmoniana . Plant Cell Tiss Organ Cult 121, 1–10 (2015). https://doi.org/10.1007/s11240-014-0683-1

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