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In vitro cultures of Sorbus aucuparia sustain replication of European mountain ash ringspot-associated virus (EMARaV)

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Abstract

The European mountain ash ringspot-associated virus (EMARaV), the type member of the newly established genus Emaravirus, causes the serious ringspot disease in Sorbus aucuparia (S.a.). Since up to now the virus could not be mechanically transmitted to herbaceous host plants, studies on its replication and gene expression strategies are hampered. Therefore, tissue cultures were established from shoots of EMARaV-infected S.a., and long-term replication of EMARaV was analyzed by detection of viral RNA3 through reverse transcription-polymerase chain reaction. EMARaV infection of calli and cell suspension cultures was detectable even 18 months after callus induction. Neither growth characteristics nor morphological appearance of cells and calli were affected by EMARaV-infection. These cultured cells should facilitate the isolation and analysis of virus particles, which was not achieved yet from S.a. leaf tissue.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

EMARaV:

European mountain ash ringspot associated virus

IBA:

Indol-3-butyric acid

MOPS:

3-(N-morpholino) propanesulfonic acid

PGR:

Plant growth regulators

PSTV:

Potato spindle tuber viroid

PVP-40:

Polyvinylpyrrolidone 40000

RT-PCR:

Reverse transcription-polymerase chain reaction

S.a :

Sorbus aucuparia L.

TMV:

Tobacco mosaic virus

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Acknowledgments

The authors thank Heidrun Meyer for her help and advice throughout this study and Dr. Hanny Tantau for critically reading the manuscript. This work was generously supported by Deutsche Forschungsgemeinschaft through project Mu 559/8–7.

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Authors and Affiliations

  1. Biocenter Klein Flottbek, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany

    Dagmar Svensson, Claudia Hirschfeld & Hans-Peter Mühlbach

  2. University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

    Rashida Perveen

  3. Division Phytomedicine, Department of Crop and Animal Sciences, Humboldt Universität zu Berlin, Lentze-allee 55/57, 14195, Berlin, Germany

    Jenny Robel

Authors
  1. Dagmar Svensson
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  2. Rashida Perveen
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  3. Claudia Hirschfeld
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  4. Jenny Robel
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  5. Hans-Peter Mühlbach
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Correspondence to Hans-Peter Mühlbach.

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Svensson, D., Perveen, R., Hirschfeld, C. et al. In vitro cultures of Sorbus aucuparia sustain replication of European mountain ash ringspot-associated virus (EMARaV). Plant Cell Tiss Organ Cult 119, 441–445 (2014). https://doi.org/10.1007/s11240-014-0535-z

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