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Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.

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Abstract

Artemisia annua L. is a commercial source of artemisinin. Nevertheless, artemisinin content within the plant is relatively low and varies depending on genotype and environment. To broaden the genetic variability, the mutation effect of 12C-ion beam irradiation on A. annua was examined. Irradiation at 2.5 Gy had a slight lethal effect to nodal segments while a noticeable lethal effect was observed at 5 and 10 Gy. Furthermore, at higher doses (20 and 50 Gy), a severe lethal effect was observed. Mutations at the DNA level of axillary bud-derived shoots were performed by RAPD. The mutation frequency at 10 Gy was about 1.7 and 2.1 times higher than that at 2.5 and 5 Gy, respectively. After growth and artemisinin production observation of 72 irradiated mutants, around 14 and 7 % of them showed higher artemisinin content and artemisinin yield compared to the controls, respectively. The highest artemisinin content in a mutant was 1.43 % DW, which was 3.2-fold higher than the original wild type. Additionally, the highest artemisinin yield in mutants was 3.68 mg/plant, which was around 1.4-fold higher than in the wild type. Moreover, irradiated mutants exhibited antibacterial activity against S. aureus, but the wild types did not. This study presents an effective application of heavy ion beam irradiation to create variations and improve artemisinin production in A. annua.

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Acknowledgments

The authors would like to thank the Office of the Higher Education Commission, Thailand for their support by providing support under the Strategic Scholarships for Frontier Research Network program for the Joint Ph.D. Program Thai Doctoral degree. We owe our sincere gratitude to the Graduate School of Science and Technology, Niigata University, Japan for financial assistance under the Global Circus Project during research at Niigata University, Japan. Thanks also due to Faculty of Science, Mahidol University, Thailand for facilities and research grant.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi District, Bangkok, 10400, Thailand

    Phithak Inthima & Kanyaratt Supaibulwatana

  2. Faculty of Agriculture, Niigata University, 2-8050, Ikarashi, Niigata, 9502181, Japan

    Phithak Inthima, Masahiro Otani & Masaru Nakano

  3. Ion Beam Breeding Laboratory, RIKEN Innovation Center, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Tomoya Hirano & Tomoko Abe

  4. Radiation Biology Team, RIKEN Nishina Accelerator Research Center, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yoriko Hayashi & Tomoko Abe

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  1. Phithak Inthima
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  2. Masahiro Otani
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  3. Tomoya Hirano
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  4. Yoriko Hayashi
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  5. Tomoko Abe
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  6. Masaru Nakano
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Correspondence to Masaru Nakano or Kanyaratt Supaibulwatana.

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Inthima, P., Otani, M., Hirano, T. et al. Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.. Plant Cell Tiss Organ Cult 119, 131–139 (2014). https://doi.org/10.1007/s11240-014-0519-z

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  • DOI: https://doi.org/10.1007/s11240-014-0519-z

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