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A reliable methodology for assessing the in vitro photosynthetic competence of two Brazilian savanna species: Hyptis marrubioides and Hancornia speciosa

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Abstract

Evaluation of photosynthetic efficiency is critical for studies on plant responses to environmental conditions as well as for genotype selection; however, there is a lack of reliable and functional protocols for such assessments of plants cultured in vitro. In this study, we aimed to adapt the conventional methodology for measuring gas exchange of plants grown in vitro to analyze the effects of irradiance, flow rate, and air humidity on the photosynthetic rate in cultured plantlets of two ‘Cerrado’ species, namely Hyptis marrubioides and Hancornia speciosa plantlets. Chlorophyll (chl) a fluorescence and chloroplastidic pigment content were also assessed. The highest photosynthetic rates were observed at a photon flux density of 600 μmol m−2 s−1, with tube inlet airflow rates between 100 and 300 mL min−1 and 80 % relative humidity in the inlet air. The electron transport rate curve, by means of chl a fluorescence, was similar to the photosynthetic rate response curve obtained with the infrared gas analyzer. These results demonstrate that both H. marrubioides and H. speciosa seedlings grown in vitro have a functional photosynthetic apparatus and respond to variations in measurement conditions, exhibiting substantial rates of CO2 assimilation under saturating irradiance conditions. The methodology proposed here can be adapted and applied to other species growing in vitro.

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Acknowledgments

The authors would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Instituto Federal Goiano—Rio Verde Campus, GO, for financial support. They are also grateful to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship provided to the master’s student involved in the research and to Prof. Francisco de Almeida Lobo, Universidade Federal do Mato Grosso, Brazil, for valuable suggestions regarding the methods applied in the present study.

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The authors declare that there are no conflict of interests.

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Authors and Affiliations

  1. Instituto Federal de Educação, Ciência e Tecnologia Goiano, Campus Rio Verde, Rodovia Sul Goiana, km 01, Zona Rural, Caixa Postal 66, Rio Verde, GO, 75901-970, Brazil

    Alan Carlos Costa, Márcio Rosa, Clarice Aparecida Megguer, Fabiano Guimarães Silva & Flávia Dionisio Pereira

  2. Departamento de Biologia Vegetal, Ed. CCBII, Av. P. H. Rolfs s/n, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-900, Brazil

    Wagner Campos Otoni

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  1. Alan Carlos Costa
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  2. Márcio Rosa
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  3. Clarice Aparecida Megguer
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  4. Fabiano Guimarães Silva
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Correspondence to Alan Carlos Costa.

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Costa, A.C., Rosa, M., Megguer, C.A. et al. A reliable methodology for assessing the in vitro photosynthetic competence of two Brazilian savanna species: Hyptis marrubioides and Hancornia speciosa . Plant Cell Tiss Organ Cult 117, 443–454 (2014). https://doi.org/10.1007/s11240-014-0455-y

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