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In silico identification and characterization of putative differentially expressed genes involved in common bean (Phaseolus vulgaris L.) seed development

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Abstract

Two genotypes of common bean (Phaseolus vulgaris L.) were studied to determine the structural cause of seed abortion in this species. In the non-abortive control (wild-type, cultivar BAT93), the histological analysis revealed a classical pattern of seed development and showed coordinated differentiation of the embryo proper, suspensor, endosperm tissue and seed coat. In contrast, the ethyl methanesulfonate (EMS) mutant (cultivar BAT93) showed disruption in the normal seed development leading to embryo abortion. Aborted embryos from these degenerate seeds showed abnormalities in suspensor and cotyledons at the globular, heart, torpedo and cotyledon stages. Exploring the feasibility of incorporating the available online bioinformatics databases, we identified 22 genes revealing high homology with genes involved in Arabidopsis thaliana embryo development and expressed in common bean immature seeds. The expression patterns of these genes were confirmed by RT–PCR. All genes were highly expressed in seed tissues. To study the expression profiles of isolated genes during Phaseolus embryogenesis, six selected genes were examined by quantitative RT–PCR analysis on the developing embryos of wild-type and EMS mutant plants. All selected genes were expressed differentially at different stages of embryo development. These results could help to improve understanding of the mechanism of common bean embryogenesis.

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Abbreviations

DAP:

Days after pollination

EMS:

Ethyl methanesulfonate

EST:

Expressed sequence tag

RT–PCR:

Reverse transcription polymerase chain reaction

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Authors and Affiliations

  1. University of Liège-Gembloux Agro-Bio Tech, Unit of Tropical Crop Husbandry and Horticulture, Gembloux Agricultural University, Passage des Déportés 2, 5030, Gembloux, Belgium

    Ghassen Abid, André Toussaint & Jean-Pierre Baudoin

  2. Department of Life Sciences, Unit of Breeding and Biodiversity, Walloon Agricultural Research Centre, Chaussée de Charleroi, 234, 5030, Gembloux, Belgium

    Ghassen Abid, Yordan Muhovski, Jean-Marie Jacquemin & Dominique Mingeot

  3. Department of Agronomy and Plant Biotechnology, Laboratory of Agronomy, University of Carthage, National Agronomy Institute of Tunisia (INAT), Avenue Charles Nicolle, 43, 1082, Tunis-Mahrajène, Tunisia

    Khaled Sassi

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  1. Ghassen Abid
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  2. Yordan Muhovski
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  3. Jean-Marie Jacquemin
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  4. Dominique Mingeot
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  5. Khaled Sassi
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  6. André Toussaint
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  7. Jean-Pierre Baudoin
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Correspondence to Ghassen Abid.

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Abid, G., Muhovski, Y., Jacquemin, JM. et al. In silico identification and characterization of putative differentially expressed genes involved in common bean (Phaseolus vulgaris L.) seed development. Plant Cell Tiss Organ Cult 107, 341–353 (2011). https://doi.org/10.1007/s11240-011-9986-7

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