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Expression of chalcone synthase influences flavonoid content and frequency of rhizogenesis in microshoots of Juglans regia L.

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Abstract

Adventitious rooting of proliferating shoots is controlled by many physical and chemical factors including phenolic and flavonoid compounds. Chalcone synthase (CHS) is an important enzyme in allocating significant amounts of carbon in the phenypropanoid pathway into the biosynthesis of flavonoid compounds. In this study, the expression of CHS gene during rhizogenesis of in vitro-grown shoots of two genotypes of walnut, ‘Sunland’ and ‘Howard’, was investigated using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The amounts of flavonoids, flavonols, proanthocyanidins and auxin in these cultures were also determined. Patterns of CHS gene expression in different genotypes of shoots undergoing rhizogenesis were associated with observed flavonoids content. Similar observations were also noted for flavonol and proanthocyanidin contents in ‘Howard’ walnut. Overall, a high frequency of rhizogenesis was accompanied with low flavonoid content in shoots. These observed relationships suggested that chalcone synthase is involved in control of flavonoid biosynthesis and in promoting rhizogenesis in walnut microshoots.

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References

  • Akkol EK, Goger F, Kosar M, Can Baser KH (2008) Phenolic composition and biological activities of Salvia halophila and Salvia virgata from Turkey. Food Chem 108:942–949

    Article  CAS  Google Scholar 

  • Bisbis B, Kevers C, Crovecoeur M, Dommes J, Gaspar T (2003) Restart of lignifications in micropropagated walnut shoots coincides with rooting induction. Biol Plant 47(1):1–5

    Article  CAS  Google Scholar 

  • Blakesley D (1994) Auxin metabolism and adventitious root initiation. In: Davis TD, Haissig BE (eds) Biology of adventitious root formation. Plenum Press, New York, pp 143–154

    Google Scholar 

  • Boonsnongcheep P, Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tissue Organ Cult 101:119–126

    Article  CAS  Google Scholar 

  • Buer CS, Imin N, Djordjevic MA (2010) Flavonoids: new roles for old molecules. J Integr Plant Biol 52:98–111

    Article  PubMed  CAS  Google Scholar 

  • Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182

    CAS  Google Scholar 

  • Cheniany M, Ebrahimzadeh H, Masoudi-nejad A, Vahdati K, Leslie Ch (2010) Effect of endogenous phenols and some antioxidant enzyme activities on rooting of Persian walnut (Juglans regia L.). Afr J Plant Sci 4(11):479–487

    CAS  Google Scholar 

  • Claudot AC, Jay-Allemand C, Megel EA, Drouet A (1993) Phenylalanine ammonia-lyase, chalcone synthase and poly phenolic compounds in adult and rejuvenated walnut trees. Trees 7:92–97

    Article  Google Scholar 

  • Claudot A-C, Jouanin L, Ernst D, Sandermann H, Drouet A (1994) Expression of chalcone synthase gene in walnut. Acta Hortic 381:203–205

    CAS  Google Scholar 

  • Claudot A-C, Ernst D, Sandermann H, Drouet A (1999) Cloning and characterization of two members of the chalcone synthase gene family from walnut. Plant Physiol Biochem 37(10):721–730

    Article  CAS  Google Scholar 

  • Curir P, Van Sumere CF, Termini A, Barthe P, Marchesini A, Dolci M (1990) Flavonoid accumulation is correlated with adventitious roots formation in Eucalyptus gunnii Hook micropropagated through axillary bud stimulation. Plant Physiol 92:1148–1153

    Article  PubMed  CAS  Google Scholar 

  • Davis TD, Haissig BE (1994) A historical evaluation of adventitious rooting research to 1993. In: Davis TD, Haissig BE (eds) Biology of adventitious root formation. Basic life sciences, vol 62. Plenum Press, New York, pp 274–335

    Google Scholar 

  • Driver JA, Kuniyuki AH (1984) In vitro propagation of Paradox walnut rootstock. Hortic Sci 19:507–509

    Google Scholar 

  • Durbin ML, McCaig B, Clegg MT (2000) Molecular evolution of chalcone synthase multigene family in the morning glory genome. Plant Mol Biol 42:79–92

    Article  PubMed  CAS  Google Scholar 

  • Ebrahimzadeh H, Abrishamchi P (2001) Changes in IAA, phenolic compounds, peroxidase, IAA oxidase, and polyphenoloxidase in relation to flower formation in Crocus sativus. Russ J Plant Physiol 48(2):190–195

    Article  CAS  Google Scholar 

  • El-Euch C, Jay-Allemand C, Pastiglia M, Doumas P, Charpentier JP, Capelli P, Jouanin L (1998) Expression of antisense chalcone synthase RNA in transgenic hybrid walnut microcuttings: effect on flavonoid content and rooting ability. Plant Mol Biol 38:467–479

    Article  PubMed  CAS  Google Scholar 

  • Estrada-Zúñiga ME, Cruz-Sosa F, Rodríguez-Monroy M, Verde-Calvo JR, Vernon-Carter EJ (2009) Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth. Plant Cell Tissue Organ Cult 97:39–47

    Article  Google Scholar 

  • Fogaca CM, Fett-Neto AG (2005) Role of auxin and its modulators in the adventitious rooting of Eucalyptus species differing in recalcitrance. Plant Growth Regul 45:1–10

    Article  CAS  Google Scholar 

  • Gaspar T, Kevers C, Hausman JF, Ripetti V (1994) Peroxidase activity and endogenous free auxin during adventitious root formation. In: Lumsden PJ, Nicholas JR, Davies WJ (eds) Physiology, growth and development of plants in culture. Kluwer, Dordrecht, pp 289–298

    Chapter  Google Scholar 

  • Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol 40:347–369

    Article  CAS  Google Scholar 

  • Harborne JB, Williams C (2000) Advances in flavonoids research since 1992. Phytochemistry 5:481–504

    Article  Google Scholar 

  • Heilor MC, Kevers C, Hausman JF, Gaspar T (1996) Changes in the concentrations of auxins and polyamines during rooting of in vitro propagated walnut shoots. Tree Physiol 16(5):515–519

    Google Scholar 

  • Ito M, Ichinose Y, Kato H, Shiraishi T, Yamada T (1997) Molecular evolution and functional relevance of the chalcone synthase genes of pea. Mol Gen Genet 255:28–37

    Article  PubMed  CAS  Google Scholar 

  • Jay-Allemand C, Capelli P, Cornu D (1992) Root development of in vitro hybrid walnut microcuttings in a vermiculite containing gelrite medium. Sci Hort 51:335–342

    Article  Google Scholar 

  • Koes RE, Quattrocchio R, Mol JNM (1994) The flavonoid biosynthetic pathway in plants: function and evolution. Bio Essays 16:123–132

    CAS  Google Scholar 

  • Kreuzaler F, Ragg H, Heller W, Tesch R, Witt L, Hammer D, Hahlbrock K (1979) Flavanone synthase from Petroselinum hortense. Molecular weight, subunit composition, size of messenger RNA and absence of pantetheinyl residue. Eur J Biochem 99:89–96

    Article  PubMed  CAS  Google Scholar 

  • Ksouri R, Megdiche W, Falleh H, Trabelsi N, Boulaaba M (2008) Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. C R Biol 331:865–873

    Article  PubMed  CAS  Google Scholar 

  • Kubasek WL, Ausubel FM, Shirley BW (1998) A light-independent developmental mechanism potentiates flavonoid gene expression in Arabidopsis seedlings. Plant Mol Biol 37:217–223

    Article  PubMed  CAS  Google Scholar 

  • Liu Ch, Callow P, Rowland LJ, Hancock JF, Song G (2010) Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars. Plant Cell Tissue Organ Cult 103:137–144

    Article  Google Scholar 

  • Martin CR (1993) Structure, function, and regulation of the chalcone synthase. Int Rev Cytol 147:233–284

    Article  PubMed  CAS  Google Scholar 

  • Mosella-Chancel HL, Macheix JJ (1979) Le microbouturage in vitro du pêcher (Prunus persica Batsch): influences de certains composés phénoliques. CR Acad Sci Paris 289:567–570

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murphy A, Peer WA, Taiz L (2000) Regulation of auxin transport by aminopeptidases and endogenous flavonoids. Planta 211:315–324

    Article  PubMed  CAS  Google Scholar 

  • Northmore JA, Zhou V, Chuong SDX (2011) Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-011-0018-4

  • Paul S, Kundu A, Pal A (2011) Identification and validation of conserved microRNAs along with their differential expression in roots of Vigna unguiculata grown under salt stress. Plant Cell Tissue Organ Cult 105:233–242

    Article  CAS  Google Scholar 

  • Peer WA, Murphy AS (2006) Flavonoid as signal molecules: targets of flavonoid action. In: Grotewold E (ed) The science of flavonoids. The Ohio State University, Columbus, pp 239–268

    Chapter  Google Scholar 

  • Rios DG, Gea MA, Revilla MA, Rodriguez R (1999) Rooting responses in relation with PO, PPO and IAA-O activities on walnut (Juglans regia L.) explants. Acta Hortic 442:241–249

    Google Scholar 

  • Ripetti V, Kevers C, Gaspar T (1994) Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Adv Hortic Sci 8:29–32

    Google Scholar 

  • Shashi PJ, Rustagi A, Agnihotri PK, Kulkarni VM, Bhat V (2011) Efficient Agrobacterium-mediated transformation of Pennisetum glaucum (L.) R. Br. using shoot apices as explants source. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-011-0001-0

  • Shirley BW (1996) Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway. Trends Plant Sci 1:377–382

    Google Scholar 

  • Singh K, Kumar S, Rani A, Gulati A, Singh Ahuja P (2009) Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Funct Integr Genomics 9:125–134

    Article  PubMed  CAS  Google Scholar 

  • Sun B, Richardo-da-Silvia JM, Spranger I (1998) Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem 46:4267–4274

    Article  CAS  Google Scholar 

  • Vahdati K, Martinez-Gomez P, Leslie C, Mcgranahan G (2001) Rooting ability of walnut cultivars and peroxidase analysis. Hortic Sci 36(3):458–464

    Google Scholar 

  • Vahdati K, Leslie C, Zamani Z, Mcgranahan G (2004) Rooting and acclimatization of in vitro grown shoots from three mature Persian walnut cultivars. Hortic Sci 39:324–327

    Google Scholar 

  • Wingender R, Röhnig H, Höricke C, Wing D, Schell J (1989) Differential regulation of soybean chalcone synthase genes in plant defense symbiosis and upon environmental stimuli. Mol Gen Genet 218:315–322

    Article  PubMed  CAS  Google Scholar 

  • Zhu X-Y, Chai Sh-J, Chen L-P, Zhang M-F, Yu J-Q (2010) Induction and origin of adventitious roots from chimeras of Brassica juncea and Brassica oleracea. Plant Cell Tissue Organ Cult 101:287–294

    Article  Google Scholar 

  • Zia M, Mirza B, Malik SA, Chaudhary MF (2010) Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time. Plant Cell Tissue Organ Cult 103:227–236

    Article  CAS  Google Scholar 

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Acknowledgments

The financial support of this research was provided by University of Tehran, College of Science. The authors would like to thank Mrs. Atoosa Tavackoli (Department of Biology, Faculty of Science, Alzahra University) for the English editing of manuscript. We also wish to thank the anonymous reviewers for very helpful and valuable advices.

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Authors and Affiliations

  1. School of Biology, College of Sciences, University of Tehran, 14155-6455, Tehran, Iran

    Monireh Cheniany & Hassan Ebrahimzadeh

  2. Laboratory of Systems Biology and Bioinformatics (LBB), Institute of Biochemistry and Biophysics and Center of Excellence in Biomathematics, University of Tehran, Tehran, Iran

    Ali Masoudi-nejad

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  1. Monireh Cheniany
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  2. Hassan Ebrahimzadeh
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Correspondence to Monireh Cheniany.

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Cheniany, M., Ebrahimzadeh, H. & Masoudi-nejad, A. Expression of chalcone synthase influences flavonoid content and frequency of rhizogenesis in microshoots of Juglans regia L.. Plant Cell Tiss Organ Cult 109, 51–59 (2012). https://doi.org/10.1007/s11240-011-0072-y

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