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Molecular characterization of a 2-Cys peroxiredoxin induced by abiotic stress in mungbean

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Abstract

A mungbean low temperature-inducible VrPrx1 encoding 2-Cys peroxiredoxin (2-Cys Prx) was cloned by subtractive suppression hybridization. The deduced VrPrx1 amino acid sequence showed highest sequence homology to 2-Cys Prxs of Phaseolus vulgaris (95%), Pisum sativum (89%), and Arabidopsis thaliana (87%). VrPrx1 RNA and protein levels were increased by low temperature, hydrogen peroxide (H2O2), and wounding but decreased by high salinity, drought, and exogenous abscisic acid. Recombinant His-tagged VrPrx1 recombinant protein protected DNA and glutamine synthetase activity from degradation via the thiol/Fe(III) oxygen mixed-function oxidation system, and exhibited peroxidase activity to H2O2 in the presence of the reducing agent dithiothreitol (DTT) in vitro. The oxidized dimers and oligomers of the VrPrx1 recombinant protein were reduced to monomers by DTT or thioredoxin. Subcellular localization studies confirmed that VrPrx1-GFP was targeted to the plastid. To evaluate the function of VrPrx1 in planta, the antioxidant activities and photosynthetic efficiency were investigated in VrPrx1-overexpressing Arabidopsis plants. VrPrx1 ectopic expression conferred improved photosynthetic efficiency under oxidative stress conditions. Hence, mungbean VrPrx1 may play an important role in protecting the photosynthetic apparatus against oxidative and abiotic stress conditions.

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Acknowledgments

This research was supported by the Genetic Evaluation of Important Biological Resources from National Institute of Biological Resources (NIBR, 2011), the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ007970) of Rural Development Administration and Basic Science Research Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (KRF-2009-0068370 and -2009-0074413), Republic of Korea.

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  1. BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Republic of Korea

    Chang-Woo Cho, Eunsook Chung, Jee-Eun Heo, Hyun-Ah So, Hong-Kyu Choi, Doh Hoon Kim, Young Soo Chung & Jai-Heon Lee

  2. School of Biological Sciences and Technology, Chonnam Natinal University, Gwangju, 500-757, Republic of Korea

    Ho Zoon Chae

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  1. Chang-Woo Cho
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Correspondence to Jai-Heon Lee.

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Chang-Woo Cho and Eunsook Chung equally contributed.

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Cho, CW., Chung, E., Heo, JE. et al. Molecular characterization of a 2-Cys peroxiredoxin induced by abiotic stress in mungbean. Plant Cell Tiss Organ Cult 108, 473–484 (2012). https://doi.org/10.1007/s11240-011-0061-1

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