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Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

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Abstract

Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS or Heller medium containing different levels of 6-benzyladenine (BA) (0.05–2 mg l−1), 2,4-dichlorophenoxyacetic acid (2,4-D) (1–10 mg l−1), and sucrose (10–50 g l−1). Moreover, the pH of the medium was varied from 5.2–6.2. In addition, cultures were subjected to light irradiation as well as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major monoterpenoids obtained were α-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol, benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds were obtained from cell suspension cultures grown in MS medium containing 5 mg l−1 2,4-D, 1 mg l−1 BA and 30 g l−1 sucrose at pH of 5.8 with incubation in complete darkness.

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References

  • Abbasi BH, Ahmad N, Fazal H, Rashid M, Mahmood T, Fatima N (2010) Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L. Plant Cell Tiss Org Cult 102(1):129–134. doi:10.1007/s11240-010-9712-x

    Article  Google Scholar 

  • Agarwal B, Singh U, Banerjee M (1992) In vitro clonal propagation of tea (Camellia sinensis (L.) O. Kuntze)In vitro clonal propagation of tea (Camellia sinensis (L.) O. Kuntze). Plant Cell Tiss Org Cult 30:1–5

    Article  CAS  Google Scholar 

  • Aridogan BC, Baydar H, Kaya S, Demirci M, Ozbasar D, Mumcu E (2002) Antimicrobial activity and chemical composition of some essential oils. Arch Pharm Res 25(6):860–864

    Article  PubMed  CAS  Google Scholar 

  • Bae TW, Park HR, Kwak YS, Lee HY, Ryu SB (2005) Agrobacterium tumefaciens-mediated transformation of a medicinal plant Taraxacum platycarpum. Plant Cell Tiss Org Cult 80(1):51–57

    Article  CAS  Google Scholar 

  • Banthorpe DV, Bates MJ, Ireland MJ (1995) Stimulation of accumulation of terpenoids by cell suspensions of Lavandula angustifolia following pretreatment of parent callus. Phytochemistry 40(1):83–87

    Article  CAS  Google Scholar 

  • Bitam F, Gavatta ML, Manzo E, Dibi A, Gavagnin M (2008) Chemical characterisation of the terpenoid constituents of the Algerian plant Launaea arborescens. Phytochemistry 69(17):2984–2992

    Article  PubMed  CAS  Google Scholar 

  • Butenko RG (1999) Biology of higher plant cells in vitro as the basis for biotechnology. FBK-PRESS, Moscow

    Google Scholar 

  • Chen QC, Youn U, Min BS, Bae K (2008) Pyronane monoterpenoids from the fruit of Gardenia jasminoides. J Nat Prod 71(6):995–999

    Article  PubMed  CAS  Google Scholar 

  • Clark JB, Chamblee TS (1992) Acid-catalyzed reactions of citrus oils and other terpene-containing flavors. In: Charalambous G (ed) Off-flavors in food beverages. Elsevier Science, New York, pp 229–285

    Google Scholar 

  • Crowell PL (1999) Prevention and therapy of cancer by dietary monoterpenes. J Nutr 129(3):775–778

    Google Scholar 

  • Davis EM, Croteau R (2000) Cyclization enzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes. Top Curr Chem 209:53–95

    Article  CAS  Google Scholar 

  • Franco MRB, Shibamoto T (2000) Volatile composition of some Brazilian fruits: Umbu-caja (Spondias citherea), camu–camu (Myrciaria dubia), arapa-boi (Eugenia stipitata), and cupuacu (Theobroma grandiflorum). J Agr Food Chem 48(4):1263–1265

    Article  CAS  Google Scholar 

  • Girard B, Fukumoto L, Mazza G, Delaquis P, Ewert B (2002) Volatile terpene constituents in maturing Gewurztraminer grapes from British Columbia. Am J Enol Viticult 53(2):99–109

    CAS  Google Scholar 

  • Godefroot M, Sandra P, Verzele M (1981) New method for quantitative essential oil analysis. J Chromatogr 203:325–335

    Article  CAS  Google Scholar 

  • Jirovetz L, Buchbauer G, Schmidt E, Stoyanova AS, Denkova Z, Nikolova R, Geissler M (2007) Purity, antimicrobial activities and olfactoric evaluations of geraniol/nerol and various of their derivatives. J Essent Oil Res 19(3):288–291

    CAS  Google Scholar 

  • Katoh S, Croteau R (1998) Individual variation in constitutive and induced monoterpene biosynthesis in grand fir. Phytochemistry 47(4):577–582

    Article  CAS  Google Scholar 

  • Kawakami M, Ganguly SN, Banerjee J, Kobayashi A (1995) Aroma composition of oolong tea and black tea by brewed extraction method and characterizing compounds of darjeeling tea aroma. J Agr Food Chem 43(1):200–207

    Article  CAS  Google Scholar 

  • Kim TH, Shin JH, Baek HH, Lee HJ (2001) Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint). J Sci Food Agr 81(6):569–575

    Article  CAS  Google Scholar 

  • Liu K, Rossi PG, Ferrari B, Berti L, Casanova J, Tomi F (2007) Composition, irregular terpenoids, chemical variability and antibacterial activity of the essential oil from Santolina corsica Jordan et Fourr. Phytochemistry 68(12):1698–1705

    Article  PubMed  CAS  Google Scholar 

  • Liu YJ, Gao LP, Xia T, Zhao L (2009) Investigation of the site specific accumulation of catechins in the tea plant (Camellia sinensis (L.) O. Kuntze) via vanillin-HCl staining. J Agr Food Chem 57(21):10371–10376

    Article  CAS  Google Scholar 

  • Lopez MG, Sanchez-Mendoza IR, Ochoa-Alejo N (1999) Compartive study of volatile components and fatty acids of plants and in vitro cultures of parsley (Petroselinum crispum (Mill) Nym ex hill). J Agr Food Chem 47(8):3292–3296

    Article  CAS  Google Scholar 

  • Loza-Tavera H (1999) Monoterpenes in essential oils: biosynthesis and properties. Adv Exp Med Biol 464:49–62

    Article  PubMed  CAS  Google Scholar 

  • Mccaskill D, Gershenzon J, Croteau R (1992) Morphology and monoterpene biosynthetic capabilities of secretory-cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L). Planta 187(4):445–454

    Article  CAS  Google Scholar 

  • Mcdonald KA, Jackman AP (1989) Bioreactor studies of growth and nutrient utilization in alfalfa suspension-cultures. Plant Cell Rep 8(8):455–458

    Article  CAS  Google Scholar 

  • Mondal TK, Bhattacharya A, Laxmikumaran M, Ahuja PS (2004) Recent advances of tea (Camellia sinensis) biotechnology. Plant Cell Tiss Org Cult 76(3):195–254

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nabeta K, Ohnishi Y, Hirose T, Sugisawa H (1983) Monoterpene biosynthesis by callus tissues and suspension cells from Perilla species. Phytochemistry 22(2):423–425

    Article  CAS  Google Scholar 

  • Niedz RP, Moshonas MG, Peterson B, Shapiro JP, Shaw PE (1997) Analysis of sweet orange (Citrus sinensis (L.) Osbeck) callus cultures for volatile compounds by gas chromatography with mass selective detector. Plant Cell Tiss Org 51(3):181–185

    Article  CAS  Google Scholar 

  • Nikolaeva TN, Zagoskina NV, Zaprometov MN (2009) Production of phenolic compounds in callus cultures of tea plant under the effect of 2, 4-D and NAA. Russ J Plant Physl 56(1):45–49. doi:10.1134/S1021443709010075

    Article  CAS  Google Scholar 

  • Nurazah Z, Radzali M, Syahida A, Maziah M (2009) Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant. Afr J Biotechnol 8(12):2740–2743

    CAS  Google Scholar 

  • Oltramari AC, Wood KV, Bonham C, Verpoorte R, Caro MSB, Viana AM, Pedrotti EL, Maraschin RP, Maraschin M (2004) Safrole analysis by GC-MS of prototrophic (Ocotea odorifera (Vell.) Rohwer) cell cultures. Plant Cell Tiss Org 78(3):231–235

    Article  CAS  Google Scholar 

  • Omidi Y, Zare K, Nazemiyeh H, Movafeghi A, Khosrowshahli M, Motallebi-Azar A, Dadpour M (2010) Bioprocess engineering of Echium italicum (L.): induction of shikonin and alkannin derivatives by two-liquid-phase suspension cultures. Plant Cell Tiss Org Cult 100(2):157–164. doi:10.1007/s11240-009-9631-x

    Article  Google Scholar 

  • Pati PK, Kaur J, Singh P (2011) A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don. Plant Cell Tiss Org 105(3):299–307. doi:10.1007/s11240-010-9868-4

    Article  CAS  Google Scholar 

  • Predieri S, Rapparini F (2007) Terpene emission in tissue culture. Plant Cell Tiss Org Cult 91(2):87–95. doi:10.1007/s11240-007-9250-3

    Article  CAS  Google Scholar 

  • Pretto FR, Santarem ER (2000) Callus formation and plant regeneration from Hypericum perforatum leaves. Plant Cell Tiss Org 62(2):107–113

    Article  CAS  Google Scholar 

  • Ravichandran R, Parthiban R (1998) Occurrence and distribution of lipoxygenase in Camellia sinensis (L.) O Kuntze and their changes during CTC black tea manufacture under southern Indian conditions. J Sci Food Agr 78(1):67–72

    Article  CAS  Google Scholar 

  • Rawat R, Gulati A, Babu GDK, Acharya R, Kaul VK, Singh B (2007) Characterization of volatile components of Kangra orthodox black tea by gas chromatography-mass spectrometry. Food Chem 105(1):229–235

    Article  CAS  Google Scholar 

  • Sahai OM (1994) Plant tissue culture. In: Gabelman A (ed) Bioprocess production of flavour, fragrances and color ingredients. Wiley, New York

    Google Scholar 

  • Sandal I, Bhattacharya A, Ahuja PS (2001) An efficient liquid culture system for tea shoot proliferation. Plant Cell Tiss Org Cult 65(1):75–80

    Article  CAS  Google Scholar 

  • Santos AV, Arrigoni-Blank MF, Blank AF, Diniz LEC, Fernandes RMP (2011) Biochemical profile of callus cultures of Pogostemon cablin (Blanco) Benth. Plant Cell Tiss Org 107(1):35-43. doi:10.1007/s11240-011-9953-3

    Article  CAS  Google Scholar 

  • Saxena G, Banerjee S, Laiq-ur-Rahman VermaPC, Mallavarapu GR, Kumar S (2007) Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality. Plant Cell Tiss Org Cult 90(2):215–223. doi:10.1007/s11240-007-9261-0

    Article  CAS  Google Scholar 

  • Shibasaki-Kitakawa N, Takeishi J, Yonemoto T (2003) Improvement of catechin productivity in suspension cultures of tea callus cells. Biotechnol Progr 19(2):655–658. doi:10.1021/bp025539a

    Article  CAS  Google Scholar 

  • Supaibulwatana K, Banyai W, Kirdmanee C, Mii M (2010) Overexpression of farnesyl pyrophosphate synthase (FPS) gene affected artemisinin content and growth of Artemisia annua L. Plant Cell Tiss Org Cult 103(2):255–265. doi:10.1007/s11240-010-9775-8

    Article  Google Scholar 

  • Suvarnalatha G, Narayan MS, Ravishankar GA, Venkataraman LV (1994) Flavour production in plant cell cultures of basmati rice (Oryza sativa L). J Sci Food Agr 66(4):439–442

    Article  CAS  Google Scholar 

  • Wise ML, Croteau R (1999) Monoterpene biosynthesis. In: Cane DE (ed) Comprehensive natural products chemistry: Isoprenoids including carotenoids and steroids, vol 2. Elsevier Science, London, pp 97–153

    Google Scholar 

  • Zhu M, Li E, He H (2008) Determination of volatile chemical constitutes in tea by simultaneous distillation extraction, vacuum hydrodistillation and thermal desorption. Chromatographia 68(7–8):603–610

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Sudripta Das (Tea Research Association, Tocklai Experimental Station, Jorhat, Assam, India) for providing the tea plant material. This study was made possible in part through the support of a grant from Department of Biotechnology (DBT), Govt. of India to VSB and DS.

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  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Abhinav Grover, Jayashankar S. Yadav, Ranjita Biswas, Choppakatla S. S. Pavan, Punita Mishra, Virendra S. Bisaria & Durai Sundar

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  1. Abhinav Grover
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  2. Jayashankar S. Yadav
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  3. Ranjita Biswas
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  4. Choppakatla S. S. Pavan
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  7. Durai Sundar
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Correspondence to Durai Sundar.

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Grover, A., Yadav, J.S., Biswas, R. et al. Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis . Plant Cell Tiss Organ Cult 108, 323–331 (2012). https://doi.org/10.1007/s11240-011-0046-0

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  • DOI: https://doi.org/10.1007/s11240-011-0046-0

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