Skip to main content
SpringerLink
Log in

The effect of the orientation of stem segments of grapevine (Vitis vinifera) cv. Chardonnay on callus development in vitro

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Callus induction and formation were successfully achieved using stem segments of grapevine cv. Chardonnay. Nodal stem segments from in vitro plantlets and internodal segments from field-grown plants of grapevine were used. The segments were placed in an inverted and upright position in hormone-free medium or in medium supplemented with 1.1 mg/l naphthalene acetic acid and 0.45 mg/l benzyladenine. Firm, compact callus with pro-meristematic clusters was induced on the inverted segments within 10–20 days, while only small calluses and roots appeared on the upright segments. Vascular cells (xylem and phloem) were differentiated and maintained for more than 2 years in consecutive subcultures. The results show that inverting the position of grapevine stem segments in vitro is a simple method for producing firm callus with pro-meristematic clusters.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  • Annapurna D, Rathore TS (2010) Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F. Plant Cell Tissue Org Cult 101:269–277

    Article  Google Scholar 

  • Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Eur J Plant Pathol 114:397–404

    Article  Google Scholar 

  • García-Luis A, Bordón Y, Moreira-Dias JM, Molina RV, Guardiola JL (1999) Explant orientation and polarity determine the morphogenic response of epicotyl segments of troyer citrange. Ann Bot 84:715–723

    Article  Google Scholar 

  • Gueye B, Saı¨d-Ahmed H, Morcillo F, Borgel A, Sane´ D, Hilbert JL, Verdeil JL, Blervacq AS (2009a) Callogenesis and rhizogenesis in date palm leaf segments: are there similarities between the two auxin-induced pathways? Plant Cell Tissue Org Cult 98:47–58

    Article  CAS  Google Scholar 

  • Gueye B, Morcillo F, Collin M, Gargani D, Overvoorde P, Aberlenc-Bertossi F, Tranbarger TJ, Sane D, Tregear JW, Borgel A, Verdeil JL (2009b) Acquisition of callogenic capacity in date palm leaf tissues in response to 2, 4-D treatment. Plant Cell Tissue Org Cult 99:35–45

    Article  CAS  Google Scholar 

  • Infante R, Fiore N, Seibert E (2008) Preservation of grape fanleaf virus on callus culture of Vitis vinifera cv Cabernet Sauvignon. Acta Phytopath Entomol Hung 43:101–108

    Article  Google Scholar 

  • McCown BH, Lloyd G (1981) Woody plant medium (WPM)–a mineral nutrient formulation for microculture for woody plant species. Hortic Sci 16:453

    Google Scholar 

  • Newell C, Growns D, Mccom J (2006) Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) Cv. ‘Digger’ microcuttings in vitro. In Vitro Cell Dev Biol Plant 42:197–200

    Article  Google Scholar 

  • Perrin M, Gertz C, Euge`ne Masson JE (2004) High efficiency initiation of regenerable embryogenic callus from anther filaments of 19-grapevine genotypes grown worldwide. Plant Sci 167:1343–1349

    Article  CAS  Google Scholar 

  • Ramarosandratana AV, Van Staden J (2003) Tissue position, explant orientation and naphthaleneacetic acid (NAA) affect initiation of somatic embryos and callus proliferation in Norway spruce (Picea abies). Plant Cell Tissue Org Cult 74:249–255

    Article  CAS  Google Scholar 

  • Rodríguez A, Cervera M, Peris JE, Peña L (2008) The same treatment for transgenic shoot regeneration elicits the opposite effect in mature explants from two closely related sweet orange (Citrus sinensis (L) Osb) genotypes. Plant Cell Tissue Org Cult 93:97–106

    Article  Google Scholar 

  • Sachs T (1981) The control of the patterned differentiation of vascular tissues. Adv Bot Res 9:151–262

    Article  Google Scholar 

  • Sachs T (1991) Callus and tumor development. In: Barlow PW, Bray D, Green PB, Slack JMW (eds) Pattern formation in plant tissue. Cambridge University Press, Cambridge, pp 38–51

    Chapter  Google Scholar 

  • Scagliusi SMM, Vega J, Kuniyuki H (2002) Cytopathology of callus cells infected with grapevine leafroll associated virus 3. Fitopatol Bras 27:384–388

    Article  Google Scholar 

  • Sheldrake AR, Northcote DH (1968) The production of auxin by tobacco internode tissues. New Phytol 67:1–13

    Article  CAS  Google Scholar 

  • Torelli A, Soragni E, Borinato M, Branca C (2004) The expression of lesk1 morphogenetic marker along the tomato hypocotyl axis is linked to a position-dependent competence for shoot regeneration. Plant Sci 166:179–190

    Article  CAS  Google Scholar 

  • Woodward AW, Bartel B (2005) Auxine: regulation, action and interaction. Ann Bot 95:705–735

    Article  Google Scholar 

Download references

Acknowledgments

The research was supported by the Israeli Ministry of Science. The authors thank Rima Bordolay, Golan Research Institute, for the maintenance of the plantlet culture in vitro and Danny Joel, Newe-Ya’ar Research Center, ARO for histological staining, instructions and guidance.

Author information

Authors and Affiliations

  1. Golan Research Institute and Ohalo College, 12900, Katzrin, Israel

    Vered Naor

  2. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, Israel, 76100

    Meira Ziv

  3. Extension Service, Ministry of Agriculture, Ben Shemen, Israel

    Tirtza Zahavi

Authors
  1. Vered Naor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meira Ziv
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tirtza Zahavi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vered Naor.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Naor, V., Ziv, M. & Zahavi, T. The effect of the orientation of stem segments of grapevine (Vitis vinifera) cv. Chardonnay on callus development in vitro. Plant Cell Tiss Organ Cult 106, 353–358 (2011). https://doi.org/10.1007/s11240-010-9911-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-010-9911-5

Keywords

Search

Navigation