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Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae)

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Abstract

Passiflora suberosa is used in popular medicine, improvement programs, and as an ornamental plant. The goal of this study was to establish efficient protocols for plant regeneration and callus induction from nodal, internodal and leaf segments excised from in vitro-grown plants. The different morphogenetic responses were modulated by the type and concentration of plant growth regulators, according to the basal medium and light conditions. Shoot formation occurred through three pathways: (1) development of preexisting meristems, (2) direct organogenesis, and (3) indirect organogenesis. Development of preexisting meristems was observed from nodal segments (1 shoot/explant) in response to α-naphthaleneacetic acid (NAA), picloram (PIC), and 2,4-dichlorophenoxyacetic acid (2,4-D), using two basal media (MS and MSM). Direct organogenesis in this species was obtained for the first time in this work, through shoot development from internodal segments in the presence of 6-benzyladenine (BA). The highest regeneration rates were achieved on MSM medium, regardless of the BA concentration. Indirect organogenesis was achieved from all explant types on media supplemented with BA, used alone or in combination with NAA. The highest regeneration efficiency was obtained from internodal segments cultured on MSM medium plus 44.4 μM BA. Compact, friable, or mucilaginous non-morphogenic calluses were induced by thidiazuron, PIC, 2,4-D, and NAA. High-yielding friable calluses obtained on MSM medium supplemented with 28.9 μM PIC are being used for the establishment of suspension cultures and further analysis of the production of bioactive compounds.

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Acknowledgments

The authors acknowledge the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) for a doctoral scholarship and financial support. E. Mansur is a recipient of a research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Renata Garcia & Gabriela Borges

  2. Instituto de Biologia Roberto Alcantara Gomes, Núcleo de Biotecnologia Vegetal, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier no 524 PHLC sala 505, Maracanã, Rio de Janeiro, 20550-013, Brazil

    Renata Garcia, Georgia Pacheco, Erica Falcão, Gabriela Borges & Elisabeth Mansur

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  1. Renata Garcia
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  2. Georgia Pacheco
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  3. Erica Falcão
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  4. Gabriela Borges
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  5. Elisabeth Mansur
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Correspondence to Elisabeth Mansur.

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Garcia, R., Pacheco, G., Falcão, E. et al. Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae). Plant Cell Tiss Organ Cult 106, 47–54 (2011). https://doi.org/10.1007/s11240-010-9892-4

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  • DOI: https://doi.org/10.1007/s11240-010-9892-4

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