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Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in cut flower carnation

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Abstract

Carnation (Dianthus caryophyllus L.) is an important ornamental crop and serves as a model system for investigating ethylene-sensitive flower senescence. EIN2 (ethylene insensitive 2) is a central component of the ethylene signal transduction pathway in plants, but the transcriptional regulation of the EIN2 gene in response to ethylene has not yet been elucidated. We identified a cDNA clone encoding a putative EIN2-like protein (DCEIN2) from total RNA isolated from senescing carnation petals using reverse transcription-PCR and rapid amplification of cDNA ends procedures. The cDNA contained an open reading frame of 3828 bp corresponding to 1275 amino acids. The northern blot results indicated that DCEIN2 expression in both the petals and ovaries was enhanced by treatment with exogenous ethylene and sugar, respectively, and was inhibited by silver thiosulfate. In the carnation vegetative tissues, mRNAs for DCEIN2 were present in the leaves and stems, but they were not detected in the roots.

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Abbreviations

CTR:

Constitutive triple response

EIL:

Ethylene-insensitive3-like

EIN:

Ethylene insensitive

ERF:

Ethylene-responsive element-binding factor

ERS:

Ethylene response sensor

ETP:

EIN2 targeting protein

ETR:

Ethylene resistant

RTE1:

Reversion-to-ethylene sensitivity1

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Acknowledgments

This study was supported by National Natural Science Foundation of China (30800758 and 30972410), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and Fok Ying Tung Education Foundation (104031).

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Authors and Affiliations

  1. College of Horticulture, South China Agricultural University, Wushan Road 483, 510642, Guangzhou, China

    Zhaodi Fu, Huinan Wang, Juan Liu, Juanxu Liu, Jing Wang, Zhaoqi Zhang & Yixun Yu

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  1. Zhaodi Fu
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  2. Huinan Wang
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  3. Juan Liu
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  4. Juanxu Liu
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  5. Jing Wang
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  7. Yixun Yu
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Correspondence to Yixun Yu.

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Zhaodi Fu and Huinan Wang authors contributed equally to this work.

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Fu, Z., Wang, H., Liu, J. et al. Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in cut flower carnation. Plant Cell Tiss Organ Cult 105, 447–455 (2011). https://doi.org/10.1007/s11240-010-9886-2

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  • DOI: https://doi.org/10.1007/s11240-010-9886-2

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