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Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

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Abstract

Although suckers and seedlings can be used for the propagation of bromeliads, the low number of propagules and cross-variation limit their uniformity and mass cultivation. In this study, high-efficiency shoot organogenesis and plant regeneration were achieved on callus derived from petal and ovary explants of Aechmea fasciata (Bromeliaceae). Calluses were induced on half-strength Murashige and Skoog inorganic salts (1/2MS) supplemented with 1.0–1.5 mg l−1 2,4-dichlorophenoxyacetic acid in combination with 1.0 or 0.5 mg l−1 α-naphthaleneacetic acid (NAA), and shoots regenerated after transfer to 1/2MS basal medium containing the combination of 1.0 mg l−1 NAA + 0.5 mg l−1 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea. Those plantlets grown under a middle light intensity (50 μmol m−2 s−1) showed a dramatic increase in survival percentage (up to 95%) and the maximum number of newly developing roots. The plantlets that were transplanted onto pots were successfully grown in the greenhouse.

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Abbreviations

ABA:

Abscisic acid

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

2-iP:

2-Isopentenyladenine

MS:

Murashige and Skoog’s (1962) medium

NAA:

α-Naphthaleneacetic acid

TDZ:

1-Phenyl-3-(1,2,3-thiadiazol-5-yl) urea

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Authors and Affiliations

  1. Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan, ROC

    Ping-Lung Huang & Zin-Huang Liu

  2. Kaohsiung District Agricultural Research and Extension Station, Pingtung 908, Taiwan, ROC

    Ping-Lung Huang & Chi-Chu Tsai

  3. Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung 824, Taiwan, ROC

    Li-Jen Liao

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  1. Ping-Lung Huang
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  2. Li-Jen Liao
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  4. Zin-Huang Liu
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Correspondence to Li-Jen Liao.

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Huang, PL., Liao, LJ., Tsai, CC. et al. Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth. Plant Cell Tiss Organ Cult 105, 73–78 (2011). https://doi.org/10.1007/s11240-010-9843-0

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  • DOI: https://doi.org/10.1007/s11240-010-9843-0

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