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Structure and expression profiling of a novel calcium-dependent protein kinase gene PgCDPK1a in roots, leaves, and cell cultures of Panax ginseng

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Abstract

Calcium-dependent protein kinases (CDPKs) are proposed to play an essential role in plant defense responses. In this study, we aimed to define the full sequence of a CDPK gene of Panax ginseng and analyze its expression in roots, leaves, and cell cultures of P. ginseng, one of the most valuable Chinese traditional medicinal herbs. We isolated the full-length cDNA of a P. ginseng CDPK gene, which was designated PgCDPK1a. PgCDPK1a shares high sequence identity at the amino acidic level with previously reported CDPK sequences for other plant species. We analyzed PgCDPK1a expression in the leaves of wild-growing P. ginseng plants, and in the roots and leaves of cultivated P. ginseng plants growing in an open experimental nursery at a natural ginseng habitat. PgCDPK1a was more actively expressed in the young leaves of cultivated P. ginseng plants than in that of wild-growing ones. Finally, we analyzed the expression of the gene in control GV and five rolC and rolB transgenic callus cultures of P. ginseng with different levels of fresh biomass accumulation, pathogen-related gene expression, and ginsenoside production. We observed a strong positive correlation between fresh biomass accumulation of P. ginseng cell cultures and expression of the PgCDPK1a gene. There was a less clear negative correlation between the expression of pathogen-related genes and the content of ginsenosides with the PgCDPK1a expression in cell cultures of P. ginseng. Perhaps, PgCDPK1a is involved in ginseng growth, as a positive regulator.

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Abbreviations

4-CPA:

p-chlorophenoxyacetic acid

CDPK:

Calcium-dependent protein kinases

RT-PCR:

Reverse transcription polymerase chain reaction

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    CAS  PubMed  Google Scholar 

  • Asano T, Tanaka N, Yang G, Hayashi N, Komatsu S (2005) Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant Cell Physiol 46:356–366

    Article  CAS  PubMed  Google Scholar 

  • Berberich T, Kusano T (1997) Cycloheximide induces a subset of low temperature-inducible genes in maize. Mol Gen Genet 254:275–283

    Article  CAS  PubMed  Google Scholar 

  • Bulgakov VP, Veselova MV, Tchernoded GK, Kiselev KV, Fedoreyev SA, Zhuravlev YN (2005) Inhibitory effect of the Agrobacterium rhizogenes rolC gene on rabdosiin and rosmarinic acid production in Eritrichium sericeum and Lithospermum erythrorhizon transformed cell cultures. Planta 221:471–478

    Article  CAS  PubMed  Google Scholar 

  • Cheng SH, Willmann MR, Chen HC, Sheen J (2002) Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129:469–485

    Article  CAS  PubMed  Google Scholar 

  • Chico JM, Raíces M, Téllez-Iñón MT, Ulloa RM (2002) A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants. Plant Physiol 128:256–270

    Article  CAS  PubMed  Google Scholar 

  • Dubrovina AS, Kiselev KV, Veselova MV, Isaeva GA, Fedoreyev SA, Zhuravlev YN (2009) Enhanced resveratrol accumulation in rolB transgenic cultures of Vitis amurensis correlates with unusual changes in CDPK gene expression. J Plant Physiol 166:1194–1206

    Article  CAS  PubMed  Google Scholar 

  • Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C (2001) An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods 25:386–401

    Article  CAS  PubMed  Google Scholar 

  • Gorpenchenko TY, Kiselev KV, Bulgakov VP, Tchernoded GK, Bragina EA, Khodakovskaya MV, Koren OG, Batygina TB, Zhuravlev YN (2006) The Agrobacterium rhizogenes rolC-gene-induced somatic embryogenesis and shoot organogenesis in Panax ginseng transformed calluses. Planta 223:457–467

    Article  CAS  PubMed  Google Scholar 

  • Kim OT, Bang KH, Kim YC, Hyun DY, Kim MY, Cha SW (2009) Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tiss Organ Cult 98:25–33

    Article  CAS  Google Scholar 

  • Kiselev KV, Kusaykin MI, Dubrovina AS, Bezverbny DA, Zvyagintseva TN, Bulgakov VP (2006) The rolC gene induces expression of a pathogenesis-related beta-1,3-glucanase in transformed ginseng cells. Phytochemistry 67:2225–2231

    Article  CAS  PubMed  Google Scholar 

  • Kiselev KV, Dubrovina AS, Veselova MV, Bulgakov VP, Fedoreyev SA, Zhuravlev YN (2007) The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells. J Biotechnol 128:681–692

    Article  CAS  PubMed  Google Scholar 

  • Kiselev KV, Gorpenchenko TY, Tchernoded GK, Dubrovina AS, Grishchenko OV, Bulgakov VP, Zhuravlev YN (2008) Calcium-dependent mechanism of somatic embryogenesis in Panax ginseng cell cultures expressing the rolC oncogene. Mol Biol 42:243–252

    Article  CAS  Google Scholar 

  • Kiselev KV, Turlenko AV, Zhuravlev YN (2009) CDPK gene expression in somatic embryos of Panax ginseng expressing rolC. Plant Cell Tiss Organ Cult 99:141–149

    Article  CAS  Google Scholar 

  • Kiselev KV, Grishchenko OV, Zhuravlev YN (2010) CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng. Biol Plant 54:617–626

    Article  Google Scholar 

  • Lecourieux D, Ranjeva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytol 171:249–269

    Article  CAS  PubMed  Google Scholar 

  • Lee SS, Cho HS, Yoon GM, Ahn JW, Kim HH, Pai HS (2003) Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. Plant J 33:825–840

    Article  CAS  PubMed  Google Scholar 

  • Lévy J, Bres C, Geurts R, Chalhoub B, Kulikova O, Duc G, Journet EP, Ané JM, Lauber E, Bisseling T, Dénarié J, Rosenberg C, Debellé F (2004) A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses. Science 303:1361–1364

    Article  PubMed  Google Scholar 

  • Ludwig AA, Romeis T, Jones JD (2004) CDPK-mediated signalling pathways: specificity and cross-talk. J Exp Bot 55:181–188

    Article  CAS  PubMed  Google Scholar 

  • Monroy AF, Dhindsa RS (1995) Low-temperature signal transduction: induction of cold acclimation-specific genes of alfalfa by calcium at 25°C. Plant Cell 7:321–331

    Article  CAS  PubMed  Google Scholar 

  • Persiyanova EV, Kiselev KV, Bulgakov VP, Timchenko NF, Chernoded GK, Zhuravlev YN (2008) Defense response mechanisms of ginseng callus cultures induced by Yersinia pseudotuberculosis, a human pathogen. Rus J Plant Physiol 55:748–755

    Article  CAS  Google Scholar 

  • Romeis T, Ludwig AA, Martin R, Jones JD (2001) Calcium-dependent protein kinases play an essential role in a plant defence response. EMBO J 20:5556–5567

    Article  CAS  PubMed  Google Scholar 

  • Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K (2000) Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23:319–327

    Article  CAS  PubMed  Google Scholar 

  • Spena A, Schmülling T, Koncz C, Schell JS (1987) Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants. EMBO J 6:3891–3899

    CAS  PubMed  Google Scholar 

  • Tirichine L, Imaizumi-Anraku H, Yoshida S, Murakami Y, Madsen LH, Miwa H, Nakagawa T, Sandal N, Albrektsen AS, Kawaguchi M, Downie A, Sato S, Tabata S, Kouchi H, Parniske M, Kawasaki S, Stougaard J (2006) Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development. Nature 441:1153–1156

    Article  CAS  PubMed  Google Scholar 

  • Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Shinozaki K (1994) Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana. Mol Gen Genet 244:331–340

    Article  CAS  PubMed  Google Scholar 

  • Yoon GM, Cho HS, Ha HJ, Liu JR, Lee HS (1999) Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol Biol 39:991–1001

    Article  CAS  PubMed  Google Scholar 

  • Yue PY, Mak NK, Cheng YK, Leung KW, Ng TB, Fan DT, Yeung HW, Wong RN (2007) Pharmacogenomics and the Yin/Yang actions of ginseng: anti-tumor, angiomodulating and steroid-like activities of ginsenosides. Chin Med 2:6

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors express their thanks to Alexandra Dubrovina for the helpful comments on the manuscript. This work was supported by grants from the Far East Division of the Russian Academy of Sciences, the Grant Program “Molecular and Cell Biology” of the Russian Academy of Sciences, and the Russian Science Support Foundation.

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Authors and Affiliations

  1. Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of the Russian Academy of Sciences (FEB RAS), Stoletija Str. 159, Vladivostok, 690022, Russia

    Konstantin V. Kiselev, Anna V. Turlenko & Yuri N. Zhuravlev

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  1. Konstantin V. Kiselev
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  2. Anna V. Turlenko
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  3. Yuri N. Zhuravlev
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Correspondence to Konstantin V. Kiselev.

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Kiselev, K.V., Turlenko, A.V. & Zhuravlev, Y.N. Structure and expression profiling of a novel calcium-dependent protein kinase gene PgCDPK1a in roots, leaves, and cell cultures of Panax ginseng . Plant Cell Tiss Organ Cult 103, 197–204 (2010). https://doi.org/10.1007/s11240-010-9767-8

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  • DOI: https://doi.org/10.1007/s11240-010-9767-8

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