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Prolific shoot regeneration of Astragalus cariensis Boiss

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Abstract

Prolific shoot regeneration via organogenesis was induced from leaf and leaf petiole explants of the endemic Astragalus cariensis species on Murashige and Skoog (MS) medium with α-naphthaleneacetic acid (NAA) and benzyladenine (BA) within 8 week. The highest number of shoots (23/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. Elongated shoots were successfully rooted in MS medium with 0.5 mg/l indole-3-butyric acid. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.

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Fig. 1

Abbreviations

BA:

Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kin:

Kinetin

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthaleneacetic acid

Zea:

Zeatin

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Acknowledgements

The data presented here are part of the The Scientific and Technological Research Council of Turkey (TUBITAK) Project titled; Tissue Culture and Cytogenetic Studies in Some Endemic Astragalus L. Species (Leguminosae) for Germplasm Preservation and Agricultural Uses Project Number: TOVAG-106O136. Dr. A. Duran and Dr. M. Dinc from Selçuk University, Ahmet Keleşoğlu Education Faculty, Department of Biology Education, Konya are kindly acknowledged for the collection and supply of seeds from their natural vegetation.

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Authors and Affiliations

  1. Ahmet Keleşoğlu Education Faculty, Department of Biology Education, Selçuk University, Konya, Turkey

    Semiha Erisen

  2. Faculty of Agriculture, Department of Field Crops, Selçuk University, Konya, Turkey

    Mustafa Yorgancilar, Emine Atalay & Mehmet Babaoglu

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  1. Semiha Erisen
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  2. Mustafa Yorgancilar
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  3. Emine Atalay
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  4. Mehmet Babaoglu
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Correspondence to Semiha Erisen.

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Erisen, S., Yorgancilar, M., Atalay, E. et al. Prolific shoot regeneration of Astragalus cariensis Boiss. Plant Cell Tiss Organ Cult 100, 229–233 (2010). https://doi.org/10.1007/s11240-009-9638-3

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  • DOI: https://doi.org/10.1007/s11240-009-9638-3

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