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An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

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Abstract

In this study, an efficient protocol was developed for in vitro propagation of Pinguicula lusitanica L., a rare insectivorous plant with pharmacological value and limited reproductive capacity. The effects of two concentrations (0.1 and 0.5 mg l−1) of a range of plant growth regulators, including cytokinins (BA, KIN, and ZEA) and auxins (IAA, IBA, and NAA), and three concentrations of MS medium macronutrients (total, 1/2 and 1/4MS) on proliferation and rooting, were investigated. P. lusitanica shoots showed abundant proliferation and rooting capacity, both of which were significantly influenced by MS medium strength. The use of 1/2MS supplemented with 0.5 mg l−1 BA or KIN ensure a 29-fold rate of proliferation. Best rooting frequency and higher root number and length were attained in 1/4MS medium containing 0.2 mg l−1 IAA. Sixty percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive compounds, reducing pressure on wild stocks.

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Abbreviations

BA:

6-Benzyladenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog medium

NAA:

2-Naphthaleneacetic acid

ZEA:

Zeatin

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Acknowledgments

S. Gonçalves and T. Grevenstuk acknowledge a grant from Portuguese Science and Technology Foundation (FCT, Grant SFRH/BPD/31534/2006, Grant SFRH/BD/31777/2006, respectively).

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Authors and Affiliations

  1. Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Campus de Gambelas, 8005–139, Faro, Portugal

    Sandra Gonçalves, Ana L. Escapa, Tomás Grevenstuk & Anabela Romano

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  1. Sandra Gonçalves
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  2. Ana L. Escapa
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  3. Tomás Grevenstuk
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  4. Anabela Romano
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Correspondence to Anabela Romano.

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Gonçalves, S., Escapa, A.L., Grevenstuk, T. et al. An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant. Plant Cell Tiss Organ Cult 95, 239–243 (2008). https://doi.org/10.1007/s11240-008-9423-8

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  • DOI: https://doi.org/10.1007/s11240-008-9423-8

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