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Anthraquinones from in vitro root culture of Morinda royoc L.

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Abstract

Morinda royoc L. (Rubiaceae) root cultures were established for the production of anthraquinones (AQs). Three independent experiments were performed to evaluate the effects of different levels of indole-3-acetic acid (0–22.8 μM), culture duration (15–75 days) and subculture number (0–4). The following indicators were recorded: root fresh weight per Erlenmeyer and intracellular and extracellular AQ production. The experiments performed in this study allowed an increase of intracellular AQ content up to a maximum of 4.5 mg g−1 of fresh mass, after 30 days of culture in a medium 5.7 μM of IAA. In addition, isolation and identification of seven AQs from M. royoc L. roots is described, one of them being reported for the first time for this species. The structures of isolated compounds were determined from 1H-NMR data. To the best of our knowledge, this is the first report on AQ production from root culture of this plant.

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Abbreviations

AQ(s):

Anthraquinone(s)

FW:

Fresh weight

IAA:

Indole-3-acetic acid

MS:

Murashige–Skoog medium

NAA:

1-Naphthaleneacetic acid

TLC:

Thin layer chromatography

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Acknowledgements

This research was supported by a Fundación Carolina fellowship, a MEC grant (AGL2004-05252), and Cuban CITMA funds. The authors are grateful to Dr. José Carlos Lorenzo (Centro de Bioplantas, Cuba) for his critical reading of the manuscript.

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Authors and Affiliations

  1. Laboratorio de Ingeniería Metabólica, Centro de Bioplantas, Ciego de Avila, Cuba

    Janetsy Borroto, Maribel Rivas, Maria Blanco, Oscar Concepción, Martha Hernández & Reinaldo Trujillo

  2. Departamento de Química Orgánica Biológica, Instituto de Investigaciones Químicas y Ambientales de Barcelona, ‘‘Josep Pascual Vila’’, Consejo Superior de Investigaciones Científicas, Barcelona, Spain

    Josep Coll & Yudelsy A. Tandrón

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  1. Janetsy Borroto
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  2. Josep Coll
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  3. Maribel Rivas
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  4. Maria Blanco
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  5. Oscar Concepción
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Correspondence to Janetsy Borroto.

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Borroto, J., Coll, J., Rivas, M. et al. Anthraquinones from in vitro root culture of Morinda royoc L.. Plant Cell Tiss Organ Cult 94, 181–187 (2008). https://doi.org/10.1007/s11240-008-9403-z

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  • DOI: https://doi.org/10.1007/s11240-008-9403-z

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