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Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana

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Abstract

The gene pool of Brassica oleracea was enriched via intergeneric somatic hybridization between B. oleracea (2n = 18) and Matthiola incana (2n = 14). One hundred and eighteen plants were obtained from 96 calli. Only four plants (H1, H2, H3 and H4) showed an intermediate phenotype from the parents; among these, H1 and H3 arose from the same callus. Random amplified polymorphic DNA (RAPD), sequence-related amplified polymorphism (SRAP), and cytological analyses confirmed that H1 and H3 were hybrids. The nuclear DNA content of the regenerated plants was determined by flow cytometry. More than half of the plants contained a nuclear DNA content of 1.3 to 3.9 pg/cell, which was higher than the content of B. oleracea but lower than that of M. incana. H1 contained 4.89 ± 0.02 pg of DNA per cell, while H3 nuclear DNA content was estimated at 4.87 ± 0.06 pg/cell. Cytological study of the root-tip cells revealed that the majority of the H1 and H3 hybrid cells contained 28 chromosomes.

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Abbreviations

PEG:

polyethylene glycol

NAA:

α-naphthaleneacetic acid

BA:

6-benzylaminopurine

MS:

Murashige and Skoog basal medium

RAPD:

random amplified polymorphic DNA

SRAP:

sequence-related amplified polymorphism

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Acknowledgements

Especial thankfulness is giving to Dr. Ulrich Ryschka for our initiation in the somatic hybridization researches. This study was financially supported by the Specialized Research Found for the Doctoral Program (SRFDP) of Higher Education of the Chinese Ministry of Education (No. 20030307020), Sino-Germany Cooperation on Agricultural Science and Technology, and the Beijing Municipal Natural Science Foundation, China (No. 5050001).

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Xiaoguang Sheng & Yuelin Zhu

  2. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100089, China

    Xiaoguang Sheng, Fan Liu, Hong Zhao, Li Zhang & Bin Chen

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  1. Xiaoguang Sheng
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  2. Fan Liu
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Correspondence to Fan Liu.

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Sheng, X., Liu, F., Zhu, Y. et al. Production and analysis of intergeneric somatic hybrids between Brassica oleracea and Matthiola incana . Plant Cell Tiss Organ Cult 92, 55–62 (2008). https://doi.org/10.1007/s11240-007-9304-6

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  • DOI: https://doi.org/10.1007/s11240-007-9304-6

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