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Efficient in vitro regeneration systems for Vaccinium species

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Abstract

Efficient protocols for shoot regeneration from leaf explants suitable for micropropagation as well as for the development of transgenic plants were developed for blueberry (Vaccinium corymbosum) and lingonberry (Vaccinium vitis-idaea) cultivars. Nodal segments were used to initiate in vitro shoot cultures of lingonberry cultivar ‘Red Pearl’ and southern highbush blueberry cultivar ‘Ozarkblue’. In order to develop an optimized regeneration procedure, different types and concentrations of plant growth regulators were tested to induce adventitious shoot regeneration on excised leaves from micropropagated shoots of both cultivars. The effect on percentage regeneration and number of shoots per explant was investigated. Results indicated that zeatin was superior to TDZ and meta-topolin in promoting adventitious shoot formation. A concentration of 20 μM zeatin was most effective in promoting shoot regeneration in both cultivars, in case of ‘Red Pearl’ along with 1 μM NAA. Shoots were either allowed to root in vitro on medium containing IBA or NAA or ex vitro in a fog tunnel. IBA was superior to NAA for induction of root development in vitro in both Vaccinium cultivars. Ex vitro rooting under high humidity was tested with cuttings from mature field-grown plants, from acclimatized tissue culture derived plants and with unrooted in vitro proliferated shoots planted directly. It was found that in vitro shoots rooted better under fog than cuttings from the other plant sources and rooting was equivalent to that achieved in vitro.

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Abbreviations

IBA:

indole-3-butyric acid

NAA:

naphtaleneacetic acid

TDZ:

thidiazuron

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Acknowledgments

We thank Dierking Beerenobst Company, Germany, for providing the plant material and supporting the work.

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Author notes

  1. Melanie Schwab

    Present address: Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

Authors and Affiliations

  1. Institute of Biological Production Systems, Fruit Science Section, Gottfried Wilhelm Leibniz University of Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany

    Julia Meiners & Iris Szankowski

Authors
  1. Julia Meiners
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  2. Melanie Schwab
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  3. Iris Szankowski
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Correspondence to Iris Szankowski.

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Meiners, J., Schwab, M. & Szankowski, I. Efficient in vitro regeneration systems for Vaccinium species . Plant Cell Tiss Organ Cult 89, 169–176 (2007). https://doi.org/10.1007/s11240-007-9230-7

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