Abstract
Embryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster’ litchi trees on B5 medium containing 400 mg l−1 glutamine, 200 mg l−1 casein hydrolysate, 30 g l−1 sucrose, 4.52 μM 2,4-D, 9.30 μM kinetin and 3 g l−1 gellan gum in darkness. Embryogenic cultures consisting of proembryonic cells and masses were maintained either on semi-solid MS medium supplemented with 4.52 μM 2,4-D and 0.91 μM zeatin or as embryogenic suspension cultures in liquid medium of the same composition. Maturation of somatic embryos occurred on semi-solid MS medium with 5–20% (v/v) filter-sterilized coconut water in darkness. Recovery of plants from somatic embryos was improved with 14.4 μM GA3 on half-strength MS medium with 0.2 g l−1 activated charcoal under a 16 h photoperiod provided by cool white fluorescent lights (60–80 μmol s−1 m−2). Plants have been successfully acclimatized in the greenhouse.
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References
Desai HV, Bhatt PN, Mehta AR (1986) Plant regeneration of Sapindus trifoliatus L. (soapnut) through somatic embryogenesis. Plant Cell Rep 3:190–191
DeWald SG, Litz RE, Moore GA (1989) Maturation and germination of mango somatic embryos. J Amer Soc Hort Sci 114:837–841
Ding XD, Lu LX, Chen XJ, Guan X (2000) Identifying litchi cultivars and evaluating their genetic relationships by RAPD markers. J Trop Subtrop Bot 8:49–54
Ding XD, Lu LX, Chen XJ (2001) Segregation patterns of RAPD markers in an F1 population of Litchi chinensis Sonn. Acta Hort 558:167–172
Gamborg OL, Miller RA, Ojima K (1968) Plant cell cultures. I. Nutrient requirement of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Lai Z, Chen C, Zheng L, Chen Z (2000) Somatic embryogenesis in longan (Dimocarpus longan Lour.). In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants Vol 6, Kluwer Academic Publishers, Dordrecht, pp 415–431
Litz RE (1988) Somatic embryogenesis from cultured leaf explants of the tropical tree Euphoria longan Stend. J Plant Physiol 132:190–193
Litz RE, Raharjo SHT (2005) Dimocarpus longan longan and Litchi chinensis litchi. In: Litz RE (ed) Biotechnology of fruit and nut crops, CABI, Wallingford, pp 628–636
Litz RE, Raharjo S, Matsumoto K, Witjaksono (2005) Biotechnology. In: Menzel CM, Waite GK (eds) Longan and litchi botany production and uses, CABI, Wallingford, pp 49–48
Matsumoto K, Raharjo SHT, Dhekney S, Moon PA, Litz RE (2004) Criopreservação e embriogênese somática de calos de Dimocarpus longan (Cryopreservation and somatic embryogenesis from Dimocarpus longan calli). Pesq Agropec Brasil Brasília 39:1261–1263
Subhadrabandhu S, Stern RA (2005) Taxonomy, botany and plant development. In: Menzel CM, Waite GK (eds) Litchi and longan botany, production and uses, CABI, Wallingford, pp 25–34
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Witjaksono, Litz RE (2002) Somatic embryogenesis of avocado (Persea americana Mill.) and its application for plant improvement. Acta Hort 575:133–138
Yu CH, Chen Z (1997) Induction of litchi embryogenic calli by immature embryos and anthers culture in vitro. J Fujian Agric Univ 26:168–172
Yu CH, Chen Z (1998) Embryogenic suspension culture and protoplast isolation in lychee. Chinese J Trop Crops 19:16–21 (in Chinese with English abstract)
Yu C, Chen Z, Lu L, Lin J (2000) Somatic embryogenesis and plant regeneration from litchi protoplasts isolated from embryogenic suspensions. Plant Cell Tiss Org Cult 61:51–58
Zhou LN, Kuang ZS, Ma XJ, Chen JQ, Cao J (1996) The study of factors affecting somatic embryogenesis in young embryo culture of Litchi chinensis L. J Agric Biotech 4:161–165 (in Chinese with English abstract)
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The assistance of Ms. Pamela A. Moon is greatly appreciated. Florida Agricultural Experiment Station Journal Series No.
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Raharjo, S.H.T., Litz, R.E. Somatic embryogenesis and plant regeneration of litchi (Litchi chinensis Sonn.) from leaves of mature phase trees. Plant Cell Tiss Organ Cult 89, 113–119 (2007). https://doi.org/10.1007/s11240-007-9219-2
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DOI: https://doi.org/10.1007/s11240-007-9219-2