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The role of BAP in somatic embryogenesis induction from seed explants of Arachis species from Sections Erectoides and Procumbentes

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Abstract

Somatic embryogenesis was induced from seed explants of Arachis archeri, A. porphyrocalix (Section Erectoides) and A. appressipila (Section Procumbentes) in response to 6-benzylaminopurine (BAP). Embryo axes first developed into single shoots in response to 4.4 μM BAP. Friable embryogenic calluses were produced from the hypocotyl region of these explants in response to different BAP concentrations. Embryonic leaflets also gave rise to friable calluses, but somatic embryos were only observed in explants of A. archeri and A. appressipila. Histological analyses revealed the presence of heart-shaped, torpedo and cotyledonary stages embryos, both as isolated and fused structures. A low frequency of embryo-to-plant conversion was achieved by inducing shoot development on medium solidified with 0.5% phytagel and supplemented with 1.5% or 3% sucrose. Rooting was induced on MS supplemented with indole-3-acetic acid (IAA).

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Fig. 1

Abbreviations

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

References

  • Baker CM, Wetzstein HY (1994) Influence of auxin type and concentration on peanut somatic embryogenesis. Plant Cell Tissue Organ Cult 36:361–368

    Article  CAS  Google Scholar 

  • Chengalrayan K, Sathaye SS, Hazra S (1997) High-frequency conversion of abnormal peanut somatic embryos. Plant Cell Rep 16:783–786

    Article  CAS  Google Scholar 

  • Distabanjong K, Geneve RL (1997) Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.). Plant Cell Rep 16:334–338

    CAS  Google Scholar 

  • Gagliardi RF, Pacheco GP, Coculilo SP, Valls JFM, Mansur E (2000) In vitro plant regeneration from seed explants of wild groundnut species (Genus Arachis, Section Extranervosae). Biodivers Conserv 9:943–951

    Article  Google Scholar 

  • Jarvis A, Ferguson ME, Williams DE, Guarino L, Jones PG, Stalker HT, Valls JFM, Pittman RN, Simpson CE, Bramel P (2003) Biogeography of wild Arachis: assessing conservation status and setting future priorities. Crop Sci 43:1100–1108

    Article  Google Scholar 

  • Kaparakis G, Alderson PG (2002) Influence of high concentrations of cytokinins on the production of somatic embryos by germinating seeds of tomato, aubergine and pepper. J Hortic Sci Biotechnol 77:186–190

    CAS  Google Scholar 

  • Lakshmanan P, Taji A (2000) Somatic embryogenesis in leguminous plants. Plant Biol 2:136–148

    Article  CAS  Google Scholar 

  • Mroginski E, Rey HY, Gonzalez AM, Mroginski LA (2004) Thidiazuron promotes in vitro plant regeneration of Arachis correntina (Leguminosae) via organogenesis. J Plant Growth Regul 23:129–134

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Rey HY, Mroginski LA (2006) Somatic embryogenesis and plant regeneration in diploid and triploid Arachis pintoi. Biol Plant 50:152–155

    Article  CAS  Google Scholar 

  • Rey HY, Scocchi AM, Gonzalez AM, Mroginski LA (2000) Plant regeneration in Arachis pintoi (Leguminosae) through leaf culture. Plant Cell Rep 19:856–862

    Article  CAS  Google Scholar 

  • Sagare AP, Lee YL, Lin TC, Chen CC, Tsay HS (2000) Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae)—a medicinal plant. Plant Sci 160:139–147

    Article  PubMed  CAS  Google Scholar 

  • Sellars RM, Southward GM, Phillips GC (1990) Adventitious somatic embryogenesis from cultured immature zygotic embryos of peanut and soybean. Crop Sci 30:408–414

    Article  CAS  Google Scholar 

  • Stalker HT, Campbell WV (1983) Resistance of wild species of peanuts to an insect complex. Peanut Sci 10:30–33

    Article  Google Scholar 

  • Suhasini K, Sagare AP, Krishnamurthy KV (1996) Study of aberrant morphologies and lack of conversion of somatic embryos of chickpea (Cicer arietinum L). In vitro Cell Dev Biol Plant 32:6–10

    Article  Google Scholar 

  • Victor JMR, Murch SJ, KrishnaRaj S, Saxena PK (1999) Somatic embryogenesis and organogenesis in peanut: the role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis. Plant Growth Regul 28:9–15

    Article  CAS  Google Scholar 

  • Vidoz ML, Rey HY, Gonzalez AM, Mroginski LA (2004) Somatic embryogenesis and plant regeneration through leaf culture in Arachis glabrata (Leguminosae). Acta Physiol Plant 26:59–66

    Google Scholar 

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Acknowledgments

The authors thank Dr. Claudia Magioli and Mario Pereira for assistance in photographs. This work was supported by CNPq and FAPERJ grants.

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Authors and Affiliations

  1. Laboratório de Micropropagação e Transformação de Plantas, UERJ, Rio de Janeiro, Brazil

    G. Pacheco, R. F. Gagliardi, L. A. Carneiro & E. Mansur

  2. Departamento de Botânica, UERJ, Rio de Janeiro, Brazil

    C. H. Callado

  3. Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil

    J. F. M. Valls

Authors
  1. G. Pacheco
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  2. R. F. Gagliardi
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  3. L. A. Carneiro
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  4. C. H. Callado
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  5. J. F. M. Valls
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  6. E. Mansur
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Corresponding author

Correspondence to E. Mansur.

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Pacheco, G., Gagliardi, R.F., Carneiro, L.A. et al. The role of BAP in somatic embryogenesis induction from seed explants of Arachis species from Sections Erectoides and Procumbentes . Plant Cell Tiss Organ Cult 88, 121–126 (2007). https://doi.org/10.1007/s11240-006-9169-0

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  • DOI: https://doi.org/10.1007/s11240-006-9169-0

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