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An efficient callus suspension culture system for triploid bermudagrass (Cynodon transvaalensis × C. dactylon) and somaclonal variations

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Abstract

An efficient callus suspension culture and regeneration system in a triploid bermudagrass (Cynodon dactylon × C. transvaalensis cv. Tifeagle) was studied in this report. Proline improved callus proliferation, but had no effect on regeneration. 0.6–1.2 mg l−1 BA improved regeneration, but higher concentrations of BA (≤1.2 mg l−1) resulted in the production of rootless plantlets. The embryogenic calli were able to proliferate continuously for at least 2 years with regeneration ability through the established suspension culture system. Observations with scanning electron microscope and light microscope showed somatic embryogenesis during the regeneration. Somaclonal variations were observed in regenerated plants. More than 2000 regenerants were screened for drought tolerance in the greenhouse, from which seven lines appeared to have increased drought tolerance relative to their parental variety. It is suggested that somaclonal variation in triploid bermudagrass offers an effective tool for its breeding.

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Abbreviations

BA:

6-Benzyladenine

BAP:

6-Benzylaminopurine

CH:

Casein hydrolysate

2,4-D:

2,4-Dichlorophenoxyacetic acid

RWC:

Relative water content

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Acknowledgements

This work was funded by grants from the Natural Science Foundation of China (30371014), Guangdong Provincial Natural Science Foundation of (032263), Transgenic Plants Production and Application Project (JY03-B-32-02), High Technology Project (2001AA244082), Guangdong Provincial Science and Technology Projects (C20302) and Guangzhou Science and Technology Projects (2002Z3-E0281) and Excellent Young Faculty Foundation of Ministry of Education.

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Authors and Affiliations

  1. Biotechnology Laboratory for Turfgrass and Forages, College of Life Science, South China Agricultural University, Guangzhou, 510642, China

    Shaoyun Lu, Zhongcheng Wang, Xinxing Peng & Zhenfei Guo

  2. Department of Plant Biology, Cook College, Rutgers University, New Brunswick, NJ, 08901, USA

    Gengyun Zhang

  3. Institute of Turfgrass Science, Beijin Forestry University, Beijing, 100083, China

    Liebao Han

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  1. Shaoyun Lu
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  2. Zhongcheng Wang
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  3. Xinxing Peng
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  4. Zhenfei Guo
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  5. Gengyun Zhang
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  6. Liebao Han
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Correspondence to Zhenfei Guo.

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Lu, S., Wang, Z., Peng, X. et al. An efficient callus suspension culture system for triploid bermudagrass (Cynodon transvaalensis × C. dactylon) and somaclonal variations. Plant Cell Tiss Organ Cult 87, 77–84 (2006). https://doi.org/10.1007/s11240-006-9138-7

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  • DOI: https://doi.org/10.1007/s11240-006-9138-7

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