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Efficient Somatic Embryogenesis in Alstroemeria

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Abstract

In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 μM thidiazuron and 0.5 μM indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 μM 2,4-dichlorophenoxy acetic acid and 2.2 μM benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 μM picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 μM BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally.

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Abbreviations

2,4-d :

2,4-dichlorophenoxy acetic acid

BA :

Benzylaminopurine

CEC :

Compact embryogenic callus

CIM :

Callus induction medium

CPM :

Callus proliferation medium

FEC :

Friable embryogenic callus

IBA :

Indole-3-butyric acid

MES :

2-(N-morpholino)ethanesulfonic acid

MS :

Murashige and Skoog (1962) medium

NAA :

α-naphthaleneacetic acid

NEC :

Nonembryogenic callus

PM :

Propagation medium

SE :

Somatic embryo

SH :

Schenk and Hildebrandt (1972) medium

SIM :

Shoot induction medium

TDZ :

N-phenyl-N′-1,2,3-thidiazo-5-yl urea or thidiazuron

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Acknowledgments

We thank Royal van Zanten (the Netherlands) who kindly provided in vitro plants of the genotype VV024. We thank Dirkjan Huigen and Bert Essenstam for taking care of the plants in the greenhouse. This research was partly financed by the Ministry of Education, Republic of Korea.

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Authors and Affiliations

  1. Laboratory of Plant Breeding, The Graduate School of Experimental Plant Sciences, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands

    J. B. Kim, C. J. J. M. Raemakers, E. Jacobsen & R. G. F. Visser

  2. Central Research Institute, Neobio Co., Ltd, #26-1 Sadong-ri, Dae-Weol Myeon, Icheon, Kyong-ki, 467-854, Korea

    J. B. Kim

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  1. J. B. Kim
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  2. C. J. J. M. Raemakers
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  3. E. Jacobsen
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  4. R. G. F. Visser
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Correspondence to C. J. J. M. Raemakers.

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Kim, J.B., Raemakers, C.J.J.M., Jacobsen, E. et al. Efficient Somatic Embryogenesis in Alstroemeria . Plant Cell Tiss Organ Cult 86, 233–238 (2006). https://doi.org/10.1007/s11240-006-9110-6

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  • DOI: https://doi.org/10.1007/s11240-006-9110-6

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