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In vitro seed culture and seedling development of Calopogon tuberosus

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Abstract

A major obstacle to native orchid production is difficulty in seed germination. Culture media and light effects on seed germination of Calopogon  tuberosus var. tuberosus, a native orchid with horticultural potential, were studied. Culture media included Knudson C, Malmgren modified terrestrial orchid, and PhytoTechnology orchid seed sowing. Effects of 8 weeks continual darkness, 8 weeks 16-h photoperiod, 2 weeks dark followed by 6 weeks 16-h photoperiod, 4 weeks dark followed by 4 weeks 16-h photoperiod, and 6 weeks dark followed by 2 weeks 16-h photoperiod were examined. Percent seed germination was highest on Knudson C after 8 weeks culture; however, seedling development was enhanced on PhytoTechnology seed sowing medium during 8 weeks culture under a 16-h photoperiod. This suggests that while KC and darkness promoted seed germination, P723 and light enhanced further seedling development. Seedlings of C. tuberosus readily acclimated to greenhouse conditions.

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Abbreviations

KC:

Knudson C

MM:

Malmgren modified terrestrial orchid medium

NAD:

nicotinamide adenine dinucleotide

NADP:

nicotinamide adenine dinucleotide phosphate

P723:

Phyto Technology orchid seed sowing medium

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Acknowledgements

The authors thank Scott Stewart, Environmental Horticulture Department, University of Florida, for his comments regarding the manuscript, as well as critiquing the experimental design and protocol. Brand names are provided for references, and the authors do not solely endorse these products.

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Authors and Affiliations

  1. Department of Environmental Horticulture, University of Florida, 110675, Gainesville, FL, 32611, USA

    Philip J. Kauth & Michael E. Kane

  2. Tropical Research and Education Center, University of Florida, 18905 SW 280th Street, Homestead, FL, 33031-3314, USA

    Wagner A. Vendrame

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  1. Philip J. Kauth
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  2. Wagner A. Vendrame
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  3. Michael E. Kane
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Correspondence to Philip J. Kauth.

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Kauth, P.J., Vendrame, W.A. & Kane, M.E. In vitro seed culture and seedling development of Calopogon tuberosus . Plant Cell Tiss Organ Cult 85, 91–102 (2006). https://doi.org/10.1007/s11240-005-9055-1

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