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TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin

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Abstract

In order to optimize shoot regeneration in Kalanchoë blossfeldiana, leaf and internode explants of seven cultivars including one inter-specific were studied. The effects of various combinations of α-naphthalene acetic acid (NAA) (0, 0.57 M) and thidiazuron (TDZ) (0, 0.45, 4.5, 22.5, 67.5 µM) on MS medium were examined. In all cultivars shoot regeneration frequency and number of shoots per explant were enhanced by increasing TDZ concentration. Supplementing the media with NAA did not improve shoot regeneration. Maximum regeneration frequency and optimum concentration of TDZ for shoot regeneration depended significantly on the cultivar. Internode explants, but not leaf explants, of some cultivars, were able to produce adventitious shoots without treatment with growth regulator.

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Abbreviations

BAP:

6-benzylaminopurine

GA3 :

gibberellic acid

MS:

Murashige & Skoog

NAA:

α-naphthalene acetic acid

TDZ:

thidiazuron

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Acknowledgements

This investigation was supported by the Iranian Ministry of Science, Research & Technology. We thank Knud Jepsen A/S, Dambrovej 53, Hinnerup, Denmark, for providing us with plants for this investigation.

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Authors and Affiliations

  1. Crop Science, Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark

    Mohsen Sanikhani, Stefan Frello & Margrethe Serek

  2. Faculty of Natural Sciences, Institute for Floriculture, Tree Nursery Science and Plant Breeding, University of Hannover, Herrenhaeuser Strasse 2, 30419, Hannover, Germany

    Margrethe Serek

Authors
  1. Mohsen Sanikhani
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  2. Stefan Frello
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  3. Margrethe Serek
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Correspondence to Stefan Frello.

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Sanikhani, M., Frello, S. & Serek, M. TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin. Plant Cell Tiss Organ Cult 85, 75–82 (2006). https://doi.org/10.1007/s11240-005-9050-6

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  • DOI: https://doi.org/10.1007/s11240-005-9050-6

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