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Plant Cell, Tissue and Organ Culture

, Volume 83, Issue 3, pp 303–310 | Cite as

Production of fertile somatic hybrids of Gossypium hirsutum + G. bickii and G. hirsutum G. stockii via protoplast fusion

  • Yuqiang Sun
  • Xianlong ZhangEmail author
  • Yichun Nie
  • Xiaoping Guo
Article

Abstract

Fertile somatic hybrids were obtained via symmetric electrofusion of protoplasts from two combinations of tetraploid cotton (G. hirsutum cv. Coker 201, AD genome) and diploid wild cottons G. bickii (G genome) and G. stockii (E genome), respectively. Observation by morphological, flow cytometric analysis, chromosome counting and RAPD analysis of the tested hybrids of Coker 201 + G. bickii and Coker 201 + G. stockii confirmed the regenerated plants as hybrid status. Cytological investigation of the metaphase root-tip cells revealed there were 78 chromosomes in the hybrids. Flow cytometric analysis showed the tested plants had a relative DNA contents close to the total DNA contents of the two parents. RAPD analysis revealed the hybrids contained specific genomic fragments from both fusion partners, further confirmed their hybridity. The morphology of the hybrids was intermediate between the two fusion partners. The hybrid plants were successfully transferred to the soil, and they bloomed and set bolls. It is sure that the new hexaploids developed by cell fusion would contribute to cotton breeding through backcrossing with the elite genotypes of G. hirsutum.

Keywords

Gossypium hirsutum G. bickii G. stockii hexaploid protoplast fusion somatic hybrid 

Abbreviations

MSB

MS basal salts (Murashige and Skoog, 1962) plus B5 vitamins (Gamborg et al., 1968)

NAA

α-naphthaleneacetic acid

PCR

polymerase chain reaction

RAPD

random amplified polymorphic DNA

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Copyright information

© Springer 2005

Authors and Affiliations

  • Yuqiang Sun
    • 1
  • Xianlong Zhang
    • 1
    Email author
  • Yichun Nie
    • 1
  • Xiaoping Guo
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityHubeiChina

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