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Morphogenesis in Isolated Microspore Cultures of Brassica juncea

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Abstract

Androgenesis is a phenomenon in which microspores are made to bypass the sexual pathway and follow the sporophytic mode of development to generate new plants without the intervention of fertilization under specialized in vitro conditions. Microspore culture provides an ideal system, with a large, relatively uniform population of haploid cells, for use in mutant selection, genetic transformation and in studies on the molecular mechanism of induction of androgenesis and embryogenesis. This paper involves a study on establishing a reproducible and efficient protocol for microspore embryogenesis in various varieties of Brassica juncea. The genotype had a pronounced effect on androgenic response in microspore cultures. The cultivar Rajat exhibited the most response, producing around 3500 embryos/100 buds. The microspores of B. juncea cv. PR-45 from ed plants maintained at a day/night temperature of 10 °C/5 °C form embryos with suspensors with varied morphology. The microspore embryos germinated to produce plants with frequencies. These plants exhibited 52% survival and 74% fertility.

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Abbreviations

ABA:

abscisic acid

DAPI:

4,6-diamidino-2-phenylindole

DMRT:

duncan’s multiple range test

GA3:

giberrelic acid

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Author notes

  1. Nidhi P. Chanana

    Present address: The Energy and Resources Institute, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, 110 007, Delhi, India

    Nidhi P. Chanana

  2. The Energy and Resources Institute, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India

    Vibha Dhawan

  3. Dayalbagh Educational Institute, 282 005, Agra, Dayalbagh, Uttar Pradesh, India

    Sant S. Bhojwani

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  1. Nidhi P. Chanana
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  2. Vibha Dhawan
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  3. Sant S. Bhojwani
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Correspondence to Nidhi P. Chanana.

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Chanana, N.P., Dhawan, V. & Bhojwani, S.S. Morphogenesis in Isolated Microspore Cultures of Brassica juncea. Plant Cell Tiss Organ Cult 83, 169–177 (2005). https://doi.org/10.1007/s11240-005-4855-x

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  • DOI: https://doi.org/10.1007/s11240-005-4855-x

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