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Phenolic Content of Microcuttings of Adult Chestnut along Rooting Induction

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Abstract

From the same adult 80-year-old tree of chestnut (Castanea sativa Mill.) 2 types of material have been taken and micropropagated. This resulted in in vitro easy-to-root microshoots (basal shoot origin - BS), and hard-to-root microshoots (crown shoot origin - CR). In these shoots, the phenolic contents were analysed at 0, 2, 5 and 8 days after in vitro rooting induction by 2 minute-dipping into an indole-3-butyric acid (IBA) solution (4.9 mM) and subsequent culture in a hormone-free rooting medium. The variation of the phenolic content along the adventitious rooting process differs between CR and BS microshoots for tannin, flavonol and elagic acid concentrations, which could be related to their differential rooting capacity.

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Abbreviations

BA:

6-benzyladenine

BS:

basal sprouts

CT:

condensed tannin

CR:

crown branches

EA:

ellagic acid derivatives

ET:

ellagic tannin

F:

flavonol

GD:

Gresshoff and Doy medium (1972)

IBA:

indole-3-butyric acid

PA:

phenolic acid derivative

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Authors and Affiliations

  1. Departamento de Producción Vexetal, Escola Politécnica Superior de Lugo, 27002, Lugo, Spain

    J.L Fernández-Lorenzo & A. Rigueiro

  2. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Apartado 122, 15080, Santiago de Compostela, Spain

    A. Ballester

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  1. J.L Fernández-Lorenzo
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  2. A. Ballester
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  3. A. Rigueiro
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Correspondence to J.L Fernández-Lorenzo.

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Fernández-Lorenzo, J., Ballester, A. & Rigueiro, A. Phenolic Content of Microcuttings of Adult Chestnut along Rooting Induction. Plant Cell Tiss Organ Cult 83, 153–159 (2005). https://doi.org/10.1007/s11240-005-4786-6

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  • DOI: https://doi.org/10.1007/s11240-005-4786-6

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