Skip to main content
SpringerLink
Log in

Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum)

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract.

Fertile regenerated plants were obtained from protoplasts via somatic embryogenesis in Coker 201 (Gossypium hirsutum L.). Protoplasts were isolated from six different explants

leaves, hypocotyls, young roots, embryogenic callus, immature somatic embryos and suspension cultures and cultured in liquid thin layer KM8P medium. Callus-forming percentage of 20–50% was obtained in protoplast cultures from embryogenic callus, immature embryos and suspension cultures, and visible callus formed within 2 months. Callus-forming percentage of 5–20% in protoplast cultures from young roots, hypocotyls and leaves, and visible callus formed in 3 months. NAA 5.371 μM/kinetin 0.929 μM was effective to stimulate protoplast division and callus formation from six explants. Percentage of callus formation in the medium with 2,4-D 0.452 μM/kinetin 0.465 μM was over 40% from suspension cultures and immature embryos, 25% from embryogenic callus and 10% from hypocotyls. Callus from protoplasts developed into plantlets via somatic embryogenesis. Over 100 plantlets were obtained from protoplasts derived from 6 explants. Ten plants have been transferred to the soil, where they all have set seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-dichloropheoxyacetic acid

FW:

fresh weight

IAA:

Indole-3-acetic acid

IBA:

indole-3-butyric acid

KM8P:

Kao and Michayluk (1975) 8p medium

MSB:

Murashige and Skoog (1962) medium plus B5 vitamins

NAA:

α-naphthaleneacetic acid

PGR:

plant growth regulators

ZT:

zeatin

References

  • SS Bhojwani JB Power EC Cocking (1977) ArticleTitleIsolation, culture and division of cotton callus protoplasts Plant Sci. Lett. 8 85–89 Occurrence Handle10.1016/0304-4211(77)90176-6

    Article  Google Scholar 

  • ZX Chen SJ Li JX Yue GL Jiao SX Liu JM She JY Wu HB Wang (1989) ArticleTitlePlantlet regeneration from protoplasts isolated from an embryogenic suspension culture of cotton (Gossypium hirsutum L.) Acta. Bot.Sin. 31 IssueID12 966–969

    Google Scholar 

  • El-Shihy OM , Evans PK (1986) Protoplast isolation, culture and plant regeneration of cotton (Gossypium barbadense L) Sixth International Congress of Plant Tissue and Cell Culture. Abstract Proc, Minnesota, pp. 52.

  • El-Shihy OM , Evans PK (1983) Protoplasts In: Potrykus IC, Harms T, Hinnen A, Hutter R, King PJ , Shillito (eds). Lect. Proc. 6th Int. Protoplast Symp. Basel, August 12–16 (pp. 24–25). Birkhauser Verlag, Basel.

  • JJ Finer RH Smith (1982) ArticleTitleIsolation and culture of protoplasts from cotton (Gossypium KlotzschianumAnderss) callus cultures Plant Sci. Lett. 26 147–151 Occurrence Handle10.1016/0304-4211(82)90085-2

    Article  Google Scholar 

  • E Firrozabady DL DeBoer (1986) ArticleTitleIsolation, culture, and cell division in cotyledon protoplasts of cotton (Gossypium hirsutum and Gossypium barbadense) Plant Cell Rep. 5 127–131 Occurrence Handle10.1007/BF00269251

    Article  Google Scholar 

  • OL Gamborg RA Miller K Ojima (1968) ArticleTitleNutrient requirements of suspension cultures of soybean root cells Exp. Cell. Res. 50 151–158 Occurrence Handle10.1016/0014-4827(68)90403-5 Occurrence Handle5650857

    Article  PubMed  Google Scholar 

  • FB Lü XL Zhang JL Liu (1999) ArticleTitleProtoplast culture and plant regeneration in upland cotton Acta Agric. Boreali-Sin. 14 IssueID1 73–78

    Google Scholar 

  • T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue culture Physiol. Plant. 15 473–497

    Google Scholar 

  • MC Peeters K Willems K R Swennen (1994) ArticleTitleProtoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers Pant Cell Rep. 13 208–211

    Google Scholar 

  • Renfroe MH, Hatwig RC ,Smith RH (1986) Isolation and culture of cotton protoplasts. Proceedings Beltwide Cotton Production Research Conferences (pp. 79–80).

  • K Saka FR Katterman JC Thomas (1987) ArticleTitleCell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.) Plant Cell Rep. 6 470–472

    Google Scholar 

  • JM She JY Wu HB Wang HY Zhou ZX Chen SJ Li JX Yue (1989) ArticleTitleSomatic embryogenesis and plant regeneration from protoplast culture of cotton (Gossypium hirsutum L) Jiangsu J. Agr. Sci. 5 IssueID4 54–60

    Google Scholar 

  • JM She JY Wu HY Zhou (1991) ArticleTitleHigh frequency of plating efficiency and plant regeneration from protoplasts of cotton (Gossypium hirsutum L.) Acta Agricult. Sin. 2 109–113

    Google Scholar 

  • YQ Sun XL Zhang YC Nie XP Guo SX Jin SG Liang (2004) ArticleTitleand characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (Gossypium klotzschianumAnderss) via electrofusion Theor. Appl. Genet. 109 472–479 Occurrence Handle10.1007/s00122-004-1663-3 Occurrence Handle15114473

    Article  PubMed  Google Scholar 

  • JC Thomas FRH Katterman (1984) ArticleTitlecontrol of spontaneous lysis of protoplasts from Gossypium hirsutumanther callus Plant Sci. Lett. 36 149–154 Occurrence Handle10.1016/0304-4211(84)90250-5

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, hina

    Yuqiang Sun, Xianlong Zhang, Chao Huang, Yichun Nie & Xiaoping Guo

Authors
  1. Yuqiang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianlong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chao Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yichun Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaoping Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xianlong Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, Y., Zhang, X., Huang, C. et al. Plant regeneration via somatic embryogenesis from protoplasts of six explants in Coker 201 (Gossypium hirsutum). Plant Cell Tiss Organ Cult 82, 309–315 (2005). https://doi.org/10.1007/s11240-005-1837-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-005-1837-y

Keywords

Search

Navigation