Skip to main content
SpringerLink
Log in

Cryopreservation of Robinia pseudoacacia

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Cryopreservation of Robinia pseudoacacia explants by vitrification achieved 78% survival following the stepwise preculture of shoot tips in (0.3 + 0.5 + 0.7 M) sucrose with a 80 min incubation in PVS2; compared to 87% survival after desiccation of explants to 30% water content, following 3 days alginate bead (with glycerol and sucrose treatments) preculture in 0.7 M sucrose.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DMSO:

dimethylsulfoxide

LN:

liquid nitrogen

MS:

Murashige and Skoog medium (Murashige and Skoog, 1962)

References

  • H Bouman GJ Klerk ParticleDe (1990) ArticleTitleCryopreservation of lily meristems Acta Hortic. 266 331–337

    Google Scholar 

  • MJ Burke (1987) The glassy state and survival of anhydrous biological systems AC Leopold (Eds) Membranes, Metabolism and Dry Organisms Cornell University Press Ithaca, NY 3583–3564

    Google Scholar 

  • MP Proft ParticleDe LJ Maene PC Debergh (1985) ArticleTitleCarbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro Physiol. Plant. 65 375–379

    Google Scholar 

  • J Dereuddre S Blandin N Hassen (1991) ArticleTitleResistence of alginate-coated shoot tips of pear tree (Pyrus communis L. cv Beurré Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardening Comptes rendues de l’académie de sciences Paris 310 317–323

    Google Scholar 

  • F Engelmann (2000) Importance of cryopreservation for the conservation of plant genetic resources F Engelmann H Takagi (Eds) Cryopreservation of Tropical Germplasm Current Research Progress and Application JIRCAS, Rome 8–20

    Google Scholar 

  • GM Fahy DR McFarlane CA Angell HT Meryman (1984) ArticleTitleVitrification as an approach to cryopreservation Cryobiology 3 407–426

    Google Scholar 

  • MT Gonzalez-Arnao F Engelmann CU Villavicencio M Morenza A Rios (2000) Cryopreservation of citrus apices using the encapsulation-dehydration technique F Engelmann H Takagi (Eds) Cryopreservation of Tropical Germplasm Current Research Progress and Application JIRCAS, Rome 217–221

    Google Scholar 

  • A Grapin D Dumet H Holota N Dorion (2003) ArticleTitleCryoreservation of Pelargonium shoot tips by encapsulation-dehydration: effects of sucrose concentration, dehydration duration and genotype Acta Hortic. 623 225–230

    Google Scholar 

  • BWW Grout (1995) Cryopreservation of excised meristems, shoot tips and somatic embryos BWW Grout (Eds) Genetic Preservation of Plant Cells in vitro Springer-Verlag Berlin 47–62

    Google Scholar 

  • Hillier J & Coombes A (1998). The Hillier Manual of Trees & Shrubs. Newton Abbot, David & Charles.

  • K Hirata P Monthana A Sakai K Miyamoto (1996) ArticleTitleCryopreservation of encapsulated shoot primordials induced in horseradish (Armoracia rusticana) hairy root cultures Plant Cell Rep. 16 469–473

    Google Scholar 

  • M Lambardi A Fabbri A Caccavale (2000) ArticleTitleCryopreservation of white poplar (Populus alba L.) by vitrification of in vitro grown shoot tips Plant Cell Rep. 19 213–218

    Google Scholar 

  • D Martinez RS Tamés MA Revilla (1999) ArticleTitleCryopreservation of in vitro-grown shoot tips of hop (Humulus lupulus L.) using encapsulation-dehydration Plant Cell Rep. 19 59–63

    Google Scholar 

  • T Matsumoto K Mochida H Itamura A Sakai (2001) ArticleTitleCryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tips Plant Cell Rep. 20 398–402

    Google Scholar 

  • T Murashige (1977) ArticleTitlePlant cell and organ cultures as horticultural practices Acta Hortic. 78 17–30

    Google Scholar 

  • T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Plant Physiol. 15 473–497

    Google Scholar 

  • T Niino K Tahiro M Suzuki S Ochuchi J Magoshi T Akihama (1997) ArticleTitleCryopreservation of in vitro grown shoot tips of cherry and sweet cherry by one-step vitrification Sci. Hortic. 70 155–163

    Google Scholar 

  • T Niino A Sakai (1992) ArticleTitleCryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry Plant Sci. 87 199–206

    Google Scholar 

  • M Pâques V Monod M Poissonnier J Dereuddre (2002) Cryopreservation of Eucalyptus sp. shoot tips by the encapsulation-dehydration procedure LE Towill YPS Bajaj (Eds) Biotechnology in Agriculture and Forestry 50 Springer-Verlag Berlin 234–245

    Google Scholar 

  • M Pâques M Poisonnier E Dumas V Monod (1997) ArticleTitleCryopreservation of dormant and non-dormant broad-leaved trees Acta Hortic. 447 491–497

    Google Scholar 

  • H Paul G Gaigny BS Sangwan-Norreel (2000) ArticleTitleCryopreservation of apple (Malus × domestica Borkh.) shoot tips following encapsulation-dehydration or encapsulation-vitrification Plant Cell Rep. 19 768–774

    Google Scholar 

  • PJ Reinhoud WM Schrijnemakers F Iren JW Kijne (1995) ArticleTitleVitrification and a heat shock treatment improve cryopreservation of tobacco cell suspension compared to two-step freezing Plant Cell Tiss. Org. 42 261–267

    Google Scholar 

  • A Sakai S Kobayashi I Oiyama (1990) ArticleTitleCryopreservation of nucellar cells of navel orange (Citrus sinensis Obs var. brasiliensis Tanaka) by vitrification Plant Cell Rep. 9 30–33

    Google Scholar 

  • MJ Seufferheld C Stushnoff P Forsline GHT Gonzalez (1999) ArticleTitleCryopreservation of cold-tender apple germplasm J. Am. Soc. Hortic. Sci. 124 612–618

    Google Scholar 

  • MA Shatnawi KA Johnson (2004) ArticleTitleCryopreservation by encapsulation-dehydration of ‘Christmas bush’ (Ceratopetalum gummiferum) shoot tips In Vitro Cell. Dev. 40 239–244

    Google Scholar 

  • H Takagi (2000) Recent developments in cryopreservation of shoot apices of tropical species F Engelman H Takagi (Eds) Cryopreservation of Tropical Germplasm Current Research Progress and Application JIRCAS, Rome 178–193

    Google Scholar 

  • H Takagi NT Thinh OM Islam T Senboku A Sakai (1997) ArticleTitleCryopreservation of in vitro grown shoot tips of taro (Colocasia esculenta Scott.) by vitrification Plant Cell Rep. 16 594–599

    Google Scholar 

  • LE Towill (1995) Cryopreservation by vitrification BWW Grout (Eds) Genetic Preservation of Plant Cells in vitro Springer-Verlag Berlin 99–111

    Google Scholar 

  • T Tsuruta Y Ishimoto T Masuoka (1998) ArticleTitleEffects of glycerol on intracellular ice formation and dehydration of onion epidermis Ann. NY Acad. Sci. 858 217–226

    Google Scholar 

  • B Vandenbussche G Wayens M Proft ParticleDe (2000) ArticleTitleCryopreservation of in vitro sugar beet (Beta vulgaris L.) shoot tips by a vitrification technique Plant Cell Rep. 19 1064–1068

    Google Scholar 

  • H Verleysen G Samyn E Bockstaele ParticleVan P Debergh (2004) ArticleTitleEvaluation of analytical techniques to predict viability after cryopreservation Plant Cell Tiss. Org. Cult. 77 11–21

    Google Scholar 

  • J Wolfe G Bryant (2001) ArticleTitleCellular cryobiology: thermodynamic and mechanical effects Int. J. Refrig. 24 438–450

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Production, Faculty of Bioscience Engineering, University Gent, Coupure links 653, 9000, Gent, Belgium

    H Verleysen, P Fernandes, I. Sánchez Pinto, E. Van Bockstaele & P Debergh

  2. Department of Plant Genetics and Breeding, CLO Gent, Caritasstraat 21, Melle, 9090 , Belgium

    H Verleysen & E. Van Bockstaele

Authors
  1. H Verleysen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Sánchez Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Van Bockstaele
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P Debergh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H Verleysen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verleysen, H., Fernandes, P., Pinto, I.S. et al. Cryopreservation of Robinia pseudoacacia. Plant Cell Tiss Organ Cult 81, 193–202 (2005). https://doi.org/10.1007/s11240-004-4957-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-004-4957-x

Keywords

Search

Navigation