Abstract
An in vitro plant regeneration protocol of Cymbidium faberi from immature seeds was established. The immature seeds of 50 days old started to form rhizomes 4 months after they were cultured on hormone free medium. The rhizomes multiplied 5 times when subcultured on the medium containing 1.0 mg l−1 α-naphthalene acetic acid (NAA) for 40 days and more than 90% of the rhizomes initiated shoots within 60 days on the media containing 0.5 or 1.0 mg l−1 NAA plus 2.0 or 5.0 mg l−1N6-benzylaminopurine (BA). Plantlets were regenerated when the shoots were planted on the basal medium amended with 1 g l−1 activated charcoal for 50 days and the plantlets grew normally after transplanting.
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Abbreviations
- BA:
-
N6-benzylaminopurine
- NAA:
-
α-naphthalene acetic acid
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Chen, Y., Liu, X. & Liu, Y. In vitro plant regeneration from the immature seeds of Cymbidium faberi. Plant Cell Tiss Organ Cult 81, 247–251 (2005). https://doi.org/10.1007/s11240-004-4956-y
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DOI: https://doi.org/10.1007/s11240-004-4956-y