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Effect of auxins on Karwinskia humboldtiana root cultures

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Abstract

Effects of auxins (IAA, IBA and NAA) on K. humboldtiana root culture cultivated in 16-h photoperiod or in dark have been observed. Light affected positively the production of biomass when cultivated on medium supplemented with NAA in 10 and 25 μ mol −1 concentrations. In the presence of IAA and IBA these values were significantly lower. The growth dynamics of root cultures depended on the auxin used. The best adventitious roots elongation and lateral roots induction on media supplemented with IBA has been ascertained. Morphological and anatomical differences in dependence on auxin used were observed. NAA supported the formation of huge callus-like mass besides mostly very short roots, especially under the light. Similarly IAA induced short roots, and IBA seems to be the most effective substance for the root elongation in this model system. NAA induced roots with larger diameter under the light compared with the other two auxins used. The reason is in the different anatomical structure of roots which was characterized by higher number of cell layers and large intercellulars in the cortex. The shape of cortical cells in the presence of IBA depended on the light conditions. Isodiametric cortical cells were present in roots cultivated in 16-h photoperiod, irregularly-shaped cells in the dark. The effect of light conditions was the smallest in the case of roots grown on IAA enriched media.

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Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Karin Kollárová, Desana Lišková & Daniela Kákoniová

  2. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, 842 15, Bratislava, Slovakia

    Alexander Lux

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  1. Karin Kollárová
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  2. Desana Lišková
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  3. Daniela Kákoniová
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  4. Alexander Lux
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Correspondence to Karin Kollárová.

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Kollárová, K., Lišková, D., Kákoniová, D. et al. Effect of auxins on Karwinskia humboldtiana root cultures. Plant Cell, Tissue and Organ Culture 79, 213–221 (2004). https://doi.org/10.1007/s11240-004-0662-z

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  • DOI: https://doi.org/10.1007/s11240-004-0662-z

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