Skip to main content
SpringerLink
Log in

Morphological and molecular data for larval stages of four species of Petasiger Dietz, 1909 (Digenea: Echinostomatidae) with an updated key to the known cercariae from the Palaearctic

  • Published:
Systematic Parasitology Aims and scope Submit manuscript

Abstract

Large-tailed echinostomatid cercariae of the genus Petasiger Dietz, 1909 (Digenea: Echinostomatidae) from the planorbid snails Gyraulus albus (Müller) and Planorbis planorbis (L.) collected in Germany and the Czech Republic and metacercariae from Gasterosteus aculeatus L. (Gasterosteiformes: Gasterosteidae) collected in Canada are characterised morphologically and molecularly. The rediae, cercariae and metacercariae are described in detail and compared with the existing data on the larval stages of Petasiger spp. Comparative molecular analyses using 28S rDNA and nad1 mitochondrial sequences supported the distinct status of four species of Petasiger. Molecular and morphological evidence for their distinction and an updated key to the known large-tailed cercariae of Petasiger from the Palaearctic are provided.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abdel-Malek, E. T. (1952). Cercaria chandleri, a new echinostome species from the snail Helisoma corpulentum in Lake Itasca, Minnesota. Transactions of the American Microscopical Society, 71, 277–281.

    Article  Google Scholar 

  • Abdel-Malek, E. T. (1953). Life history of Petasiger chandleri (Trematoda: Echinostomatidae) from the pied-billed grebe, Podilymbus podiceps podiceps, with some comments on other species of Petasiger. Journal of Parasitology, 39, 152–158.

    Article  CAS  PubMed  Google Scholar 

  • Abramoff, M. D., Magalhaes, P. J., & Ram, S. J. (2004). Image processing with ImageJ. Biophotonics International, 11, 36–42.

    Google Scholar 

  • Beaver, P. C. (1939). The morphology and life history of Petasiger nitidus Linton. Journal of Parasitology, 25, 269–276.

    Article  Google Scholar 

  • Bray, R. A., Waeschenbach, A., Cribb, T. H., Weedall, G. D., Dyal, P., & Littlewood, D. T. J. (2009). The phylogeny of the Lepocreadioidea (Platyhelminthes, Digenea) inferred from nuclear and mitochondrial genes: Implications for their systematics and evolution. Acta Parasitologica, 54, 310–329.

    Article  Google Scholar 

  • Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

    Article  CAS  PubMed  Google Scholar 

  • Faltýnková, A., Gibson, D. I., & Kostadinova, A. (2008). A revision of Petasiger Dietz, 1909 (Digenea: Echinostomatidae) and a key to its species. Systematic Parasitology, 71, 1–40.

    Article  PubMed  Google Scholar 

  • Georgieva, S., Kostadinova, A., & Skírnisson, K. (2012). The life-cycle of Petasiger islandicus Kostadinova & Skírnisson, 2007 (Digenea: Echinostomatidae) elucidated with the aid of molecular data. Systematic Parasitology, 82, 177–183.

    Article  PubMed  Google Scholar 

  • Georgieva, S., Selbach, C., Faltýnková, A., Soldánová, M., Sures, B., Skírnisson, K., & Kostadinova, A. (2013). New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data. Parasites & Vectors, 6, 64.

    Article  Google Scholar 

  • Ginetsinskaya, T. A., & Dobrovolskij, A. A. (1964). On the fauna of trematode larvae of freshwater molluscs of the Volga delta. Part II. Echinostomatid cercariae (family Echinostomatidae). Trudy Astrakhanskogo Zapovednika, 9, 64–104 (In Russian).

    Google Scholar 

  • Glöer, P. (2002). Die Süßwassergastropoden Nord- und Mitteleuropas. 2, neubearbeitete Auflage. Hackenheim: ConchBooks, 327 pp.

  • Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W., & Gascuel, O. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–321.

    Article  CAS  PubMed  Google Scholar 

  • Huelsenbeck, J. P., Ronquist, F., Nielsen, R., & Bollback, J. P. (2001). Bayesian Inference of phylogeny and its impact on evolutionary biology. Science, 294, 310–2,314.

  • Karmanova, E. M. (1971). [The life cycle of Petasiger neocomense (Trematoda, Echinostomatidae).] Trudy Gel’minthologicheskoi Laboratorii, 21, 31–35 (In Russian).

  • Khan, D. (1960). Studies on larval trematodes infecting freshwater snails in London (U.K.) and some adjoining areas. Part I. Echinostome cercariae. Journal of Helminthology, 34, 277–304.

    Article  CAS  PubMed  Google Scholar 

  • Kostadinova, A. (1997). A comparative study of cercarial chaetotaxy in two species of Petasiger Dietz, 1909 (Digenea: Echinostomatidae). Systematic Parasitology, 37, 105–110.

    Article  Google Scholar 

  • Kostadinova, A. (1999). First record of Petasiger grandivesicularis Ishii, 1935 (Digenea: Echinostomatidae) in Germany. Helminthologia, 36, 101–103.

    Google Scholar 

  • Kostadinova, A., & Chipev, N. (1992). Experimental data on the life-cycle of Petasiger grandivesicularis Ishii, 1935 (Trematoda: Echinostomatidae). Systematic Parasitology, 23, 55–65.

    Article  Google Scholar 

  • Kostadinova, A., Herniou, E. A., Barrett, J., & Littlewood, D. T. J. (2003). Phylogenetic relationships of Echinostoma Rudolphi, 1809 (Digenea: Echinostomatidae) and related genera re-assessed via DNA and morphological analyses. Systematic Parasitology, 54, 159–176.

    Article  CAS  PubMed  Google Scholar 

  • Nasir, P. (1962). Two new species of giant-tailed echinostome cercariae from Planorbis carinatus (Mull). Transactions of the American Microscopical Society, 81, 132–137.

    Article  Google Scholar 

  • Odening, K. (1962). Trematoden aus einheimischen Vögeln des Berliner Tierparks und der Umgebung von Berlin. Biologisches Zentralblatt, 81, 419–468.

    Google Scholar 

  • Odening, K. (1963). Echinostomatoidea, Notocotylata und Cyclocoelida (Trematoda, Digenea, Redioinei) aus Vögeln des Berliner Tierparks. Bijdragen tot de Dierkunde, 33, 37–60.

    Google Scholar 

  • Odening, K. (1965). Zur Trematodenfauna der einheimischen Lappentaucher und des Blesshuhns. Abhandlungen und Berichte für Naturkunde und Vorgeschichte, 11, 119–132.

    Google Scholar 

  • Odhner, T. (1910). Nordostafrikanische Trematoden, grösstenteils vom weissen Nil. I; Fascioliden. Results of the Swedish Zoological Expedition to Egypt and the White Nile. 1901 under the direction of L. A. Jägerskiöld, 23A, 1–170.

  • Olson, P. D., Cribb, T. H., Tkach, V. V., Bray, R. A., & Littlewood, D. T. J. (2003). Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology, 33, 733–755.

    Article  CAS  PubMed  Google Scholar 

  • Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D. L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M. A., & Huelsenbeck, J. P. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    Article  PubMed Central  PubMed  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2,725–2,729.

  • Tkach, V. V., Littlewood, D. T. J., Olson, P. D., Kinsella, J. M., & Swiderski, Z. (2003). Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Systematic Parasitology, 56, 1–15.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank two anonymous reviewers for their valuable comments and suggestions that improved our manuscript. We are grateful to Jana Köchling, Verena Altmann and Jessica Schwelm (University of Duisburg-Essen) and the students of the Parasitology Course 2012 (International Max Planck Research School for Evolutionary Biology, Plön, Germany) for their assistance during sampling and in the laboratory, and to Blanka Škoríková (Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic) for her kind help with the figures. Furthermore, we thank Dan I. Bolnick (University of Texas at Austin, Section of Integrated Biology, Austin, TX, USA) for providing sticklebacks from Vancouver Island. This study was supported by the Czech Science Foundation (grant P505/12/G112); the ‘Sichere Ruhr’ project as part of the Bundesministerium für Bildung und Forschung (BMBF) program ‘Sustainable Water Management’ (grant 02WRS1283). CS benefited from a Deutsche Bundesstiftung Umwelt (DBU) PhD scholarship.

Author information

Authors and Affiliations

  1. Department of Aquatic Ecology and Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, Universitätsstraße 5, D-45141, Essen, Germany

    Christian Selbach & Bernd Sures

  2. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Miroslava Soldánová, Simona Georgieva & Aneta Kostadinova

  3. Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Simona Georgieva

  4. Department of Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, August-Thienemann-Strasse 2, 24306, Plön, Germany

    Martin Kalbe

Authors
  1. Christian Selbach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Miroslava Soldánová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Simona Georgieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aneta Kostadinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Martin Kalbe
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bernd Sures
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christian Selbach.

Additional information

Christian Selbach and Miroslava Soldánová contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Selbach, C., Soldánová, M., Georgieva, S. et al. Morphological and molecular data for larval stages of four species of Petasiger Dietz, 1909 (Digenea: Echinostomatidae) with an updated key to the known cercariae from the Palaearctic. Syst Parasitol 89, 153–166 (2014). https://doi.org/10.1007/s11230-014-9513-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11230-014-9513-4

Keywords

Search

Navigation