Abstract
Comparative analysis of two optical methods—optical coherence tomography (OCT) and optical coherence microscopy (OCM)—was made for vital visualization of plant tissues in tomato (Lycopersicon esculentum Mill), spiderwort (Tradescantia pallida (Rose) D. Hunt), orach (Atriplex sp.), and leaves and seeds of medium starwort (Stellaria media L.). The obtained OCT- and OCM-images allowed the morphological and functional state of plant tissues to be assessed in vivo. A higher spatial resolution of the OCM method, as compared to OCT method, allowed plant morphological structures to be identified with greater confidence. The morphological and functional state of tissues can be monitored with a time resolution of 1–4 s in intact plants, without removing them from the habitat.
Similar content being viewed by others
Abbreviations
- OCM:
-
optical coherence microscopy
- OCT:
-
optical coherence tomography
REFERENCES
Gelikonov, V.M., Gelikonov, G.V., Gladkova, N.D., Kuranov, R.V., Nikulin, N.K., Petrova, G.A., Pochinko, V.V., Pravdenko, K.I., Sergeev, A.M., Feldstein, F.I., Khanin, Ya.I., and Shabanov, D.V., Coherence Optical Tomography of Microscopic Inhomogenities in Biological Tissues, JETP Lett., 1995, vol. 61, pp. 158–162.
Gelikonov, V.M., Gelikonov, G.V., Ksenofontov, S.Yu., Kuranov, R.V., Morozov, A.N., Myakov, A.V., Turkin, A.A., Turchin, I.V., and Shabanov, D.V., New Approaches in Broadband Fiber-Optic Interferometry for Optical Coherence Tomography, Radiophys. Quantum Electronics, 2003, vol. 46, pp. 550–564.
Gladkova, N.D., Feldstein, F.I., Gelikonov, V.M., Gelikonov, G.V., Sergeev, A.M., Khanin, Ya.I., Pochinko, V.V., Nikulin, N.K., Petrova, G.A., Leonov, V.I., Pravdenko, K.I., and Shabanov, D.V., Optical Coherence Tomography: First Steps in the Clinical Practice and Perspectives, Klin. Revmatol., 1996, no. 1, pp. 38–42.
Sergeev, A.M., Gelikonov, V.M., Gelikonov, G.V., Feldstein, F.I., Gladkova, N.D., Shahova, N.M., Snopova, L.B., Shahov, A.V., Kusnetzova, I.A., Denisenko, A.N., Pochinko, V.V., Chumakov, Y.P., and Strelzova, O.S., In Vivo Endoscopic OCT Imaging of Precancer and Cancer States of Human Mucosa, Optic. Express., 1997, vol. 1, pp. 432–440.
Hettinger, J.W., Mattozzi, M., Myers, W.R., Williams, M.E., Reeves, A., Parsons, R.L., Haskell, R.C., Petersen, D.C., Wang, R., and Medford, J.I., Optical Coherence Microscopy: A Technology for Rapid, In Vivo, Non-Destructive Visualization of Plant and Plant Cells, Plant Physiol., 2000, vol. 123, pp. 3–15.
Sapozhnikova, V.V., Kamenskii, V.A., and Kuranov, R.V., Visualization of Plant Tissue by Optical Coherence Tomography, Fiziol. Rast. (Moscow), 2003, vol. 50, pp. 316–320 (Russ. J. Plant Physiol., Engl. Transl., pp. 282–290).
Gelikonov, G.V., Gelikonov, V.M., Ksenofontov, S.U., Morosov, A.N., Myakov, A.V., Potapov, Yu.P., Saposhnikova, V.V., Sergeeva, E.A., Shabanov, D.V., Shakhova, N.M., and Zagainova, E.V., Compact Optical Coherence Microscope, Part 5 (Microscopy), Coherence-Domain Optical Methods in Biomedical Diagnostics, Environmental, and Material Science, Tuchin, V.V., Ed., Dordrecht: Kluwer, 2003, vol. 2, pp. 342–362.
Sapozhnikova, V.V., Kamensky, V.A., and Kuranov, R.V., Optical Coherence Tomography for Visualization of Plant Tissues: Laser Applications in Medicine, Biology, and Environmental Science, Proc. SPIE, 2004, vol. 5149, pp. 231–238.
Sapozhnikova, V.V., Kamensky, V.A., Kuranov, R.V., Kutis, I., Snopova, L.B., and Myakov, A.V., In Vivo Visualization of Tradescantia Leaf Tissue and Monitoring the Physiological and Morphological States under Different Water Supply Conditions Using Optical Coherence Tomography, Planta, 2004, vol. 219, pp. 601–609.
Roshchina, V.D. and Roshchina, V.V., Vydelitel’naya funktsiya vysshikh rastenii (Secretory Function in Higher Plants), Moscow: Nauka, 1989.
Dunn, A. and Richards-Kortum, R., Three-Dimensional Computation of Light Scattering from Cells, IEEE J. Select. Top. Quan. Electronics, 1996, vol. 2, pp. 898–905.
Author information
Authors and Affiliations
Additional information
__________
Translated from Fiziologiya Rastenii, Vol. 52, No. 4, 2005, pp. 628–634.
Original Russian Text Copyright © 2005 by Kutis, Sapozhnikova, Kuranov, Kamenskii.
Rights and permissions
About this article
Cite this article
Kutis, I.S., Sapozhnikova, V.V., Kuranov, R.V. et al. Study of the Morphological and Functional State of Higher Plant Tissues by Optical Coherence Microscopy and Optical Coherence Tomography. Russ J Plant Physiol 52, 559–564 (2005). https://doi.org/10.1007/s11183-005-0083-9
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11183-005-0083-9