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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

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Photorespiration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1653))

Abstract

High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.

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Author information

Authors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St., Toronto, ON, Canada, M5S 3B2

    Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis & Tammy L. Sage

Authors
  1. Roxana Khoshravesh
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  2. Vanessa Lundsgaard-Nielsen
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  3. Stefanie Sultmanis
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  4. Tammy L. Sage
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Corresponding author

Correspondence to Tammy L. Sage .

Editor information

Editors and Affiliations

  1. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Alisdair R. Fernie

  2. Plant Physiology Department, University of Rostock, Rostock, Germany

    Hermann Bauwe

  3. Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany

    Andreas P.M. Weber

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Khoshravesh, R., Lundsgaard-Nielsen, V., Sultmanis, S., Sage, T.L. (2017). Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. In: Fernie, A., Bauwe, H., Weber, A. (eds) Photorespiration. Methods in Molecular Biology, vol 1653. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7225-8_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7225-8_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7224-1

  • Online ISBN: 978-1-4939-7225-8

  • eBook Packages: Springer Protocols

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