Skip to main content
SpringerLink
Log in

Activities of Noncyclic and Alternative Pathways of Photosynthetic Electron Transport in Leaves of Broad Bean Plants Grown at Various Light Irradiances

  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Activities of noncyclic and alternative pathways of photosynthetic electron transport were studied in intact leaves of broad been (Vicia faba L.) seedlings grown under white light at irradiances of 176, 36, and 18 µmol quanta/(m2 s). Electron flows were followed from light-induced absorbance changes at 830 nm related to redox transformations of P700, the photoactive PSI pigment. The largest absorbance changes at 830 nm, induced by either white or far-red light, were observed in leaves of seedlings grown at irradiance of 176 µmol quanta/(m2 s), which provides evidence for the highest concentration of PSI reaction centers per unit leaf area in these seedlings. When actinic white light of 1800 µmol quanta/(m2 s) was turned on, the P700 oxidation proceeded most rapidly in leaves of seedlings grown at irradiance of 176 µmol quanta/(m2 s). The rates of electron transfer from PSII to PSI were measured from the kinetics of dark P700+ reduction after turning off white light. These rates were similar in leaves of all light treatments studied, and their characteristic reaction times were found to range from 9.2 to 9.5 ms. Four exponentially decaying components were resolved in the kinetics of dark P700+ reduction after leaf exposure to far-red light. A minor but the fastest component of P700+ reduction with a halftime of 30–60 ms was determined by electron transfer from PSII, while the three other slow components were related to the operation of alternative electron transport pathways. Their halftimes and relative magnitudes were almost independent on irradiance during plant cultivation. It is concluded that irradiance during plant growth affects the absolute content of PSI reaction centers in leaves but did not influence the rates of noncyclic and alternative electron transport.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ΔA 830 :

absorbance changes at 830 nm

PSI and PSII:

photosystems I and II

REFERENCES

  1. Senger, H. and Fleischacker, P.H., Adaptation of the Photosynthetic Apparatus of Scenedesmus obliquus to Strong and Weak Light Conditions: 1. Differences in Pigments, Photosynthetic Capacity, Quantum Yield and Dark Reactions, Physiol. Plant., 1978, vol. 43, pp. 35–42.

    Google Scholar 

  2. Lichtenthaler, H.K., Kuhn, G., Preuzel, U., Buschmann, C., and Melis, A., Adaptation of Chloroplast Ultrastructure and Chlorophyll-Protein Levels to High-Light and Low-Light Growth Conditions, Z. Naturforsch., 1982, vol. 37c, pp. 464–475.

    Google Scholar 

  3. Leong, T.-Y. and Anderson, J.M., Adaptation of the Thylakoid Membranes of Pea Chloroplasts to Light Intensities: 1. Study on the Distribution of Chlorophyll-Protein Complexes, Photosynth. Res., 1984, vol. 5, pp. 105–115.

    Article  Google Scholar 

  4. Boardman, N.K., Comparative Photosynthesis of Sun and Shade Plants, Annu. Rev. Plant Physiol., 1977, vol. 28, pp. 355–377.

    Article  Google Scholar 

  5. Melis, A. and Harvey, G.W., Regulation of Photosystem Stoichiometry, Chlorophyll a and Chlorophyll b Content and Relation to Chlorophyll Ultrastructure, Biochim. Biophys. Acta, 1981, vol. 637, pp. 138–145.

    Google Scholar 

  6. Markwell, J.P., Thornber, J.P., and Boggs, R.T., Higher Plant Chloroplasts: Evidence that All the Chlorophyll Exists as Chlorophyll-Protein Complexes, Proc. Natl. Acad. Sci. USA, 1979, vol. 79, pp. 1233–1235.

    Google Scholar 

  7. Paulsen, H., Chlorophyll a/b-Binding Proteins, Photochem. Photobiol., 1995, vol. 62, pp. 367–382.

    Google Scholar 

  8. Anderson, J.M., Photoregulation of the Composition, Function, and Structure of Thylakoid Membranes, Annu. Rev. Plant Physiol., 1986, vol. 37, pp. 93–136.

    Article  Google Scholar 

  9. Wild, A., Hopfner, M., Ruhle, W., and Richter, M., Changes in the Stoichiometry of Photosystem II Components as an Adaptive Response to High-Light and Low-Light Conditions during Growth, Z. Naturforsch., 1986, vol. 41c, pp. 597–603.

    Google Scholar 

  10. Leong, T.-Y. and Anderson, J.M., Adaptation of the Thylakoid Membranes of Pea Chloroplasts to Light Intensities: 2. Regulation of Electron Transport Capacities, Electron Carriers, Coupling Factor (CFI) Activity and Rates of Photosynthesis, Photosynth. Res., 1984, vol. 5, pp. 117–128.

    Article  Google Scholar 

  11. Bukhov, N.G., Rozhkovskii, A.D., Chetverikov, A.G., and Voskresenskaya, N.P., Content of Pigments, Photosynthetic Reaction Centers, and Potential Photosynthesis in Barley Seedlings Grown under Blue and Red Light with Different Intensity, Fiziol. Rast. (Moscow), 1984, vol. 31, pp. 576–584 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  12. Bjorkman, O., Boardman, N.K., Anderson, J.M., Thorne, S.W., Goodchild, D.J., and Pyliotis, N.A., Effect of Light Intensity during Growth of Atriplex patula on the Capacity of Photosynthetic Reactions, Chloroplast Components and Structure, Carnegie Inst. Yearbook, 1971, vol. 71, pp. 115–135.

    Google Scholar 

  13. Shmeleva, V.P., Ivanov, B.N., and Akulova, E.A., Photophosphorylation and Electron Transport in the Chloroplasts of Pea Grown under Light of Different Intensity, Fiziol. Rast. (Moscow), 1976, vol. 23, pp. 869–876 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  14. Nikolaeva, M.K. and Osipova, O.P., Functional Activity in the Chloroplasts of Broad Beans Grown under Light of Different Intensity, Fiziol. Rast. (Moscow), 1979, vol. 26, pp. 799–806 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  15. Mukhin, E.N., Khruslova, S.G., Egorova, E.F., and Shmeleva, V.L., Effect of Light Intensity during Plant Growth on the Rate of Ascorbate-Dependent NADP Photoreduction in Chloroplasts, Dokl. Akad. Nauk SSSR, 1970, vol. 193, pp. 940–943.

    Google Scholar 

  16. Nikolaeva, M.K. and Osipova, O.P., Ferredoxin-NADP Reductase Activity in Chloroplasts of Broad Beans Grown at Different Irradiances, Fiziol. Rast. (Moscow), 1983, vol. 30, pp. 563–569 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  17. Fork, D.C. and Herbert, S.K., Electron Transport and Photophosphorylation by Photosystem I In Vivo in Plants and Cyanobacteria, Photosynth. Res., 1993, vol. 36, pp. 149–168.

    Article  Google Scholar 

  18. Bukhov, N.G., Egorova, E.A., and Carpentier, R., Electron Flow to Photosystem I from Stromal Reductants In Vivo: The Size of the Pool of Stromal Reductants Controls the Rate of Electron Donation to Both Rapidly and Slowly Reducing Photosystem I Units, Planta, 2002, vol. 215, pp. 812–820.

    Article  PubMed  Google Scholar 

  19. Schreiber, U., Klughammer, C., and Neubauer, C., Measuring P700 Absorbance Changes around 830 nm with a New Type of Pulse Modulated System, Z. Naturforsch., 1988, vol. 43c, pp. 686–698.

    Google Scholar 

  20. Klughammer, C. and Schreiber, U., An Improved Method Using Saturating Light Pulses, for the Determination of Photosystem I Quantum Yield Via P700+-Absorbance Changes at 830 nm, Planta, 1994, vol. 192, pp. 261–268.

    Article  Google Scholar 

  21. Laisk, A., Siebke, K., Gerst, U., Eichelmann, H., Oja, V., and Heber, U., Oscillation in Photosynthesis Are Initiated and Supported by Imbalances in the Supply of ATP and NADPH to the Calvin Cycle, Planta, 1991, vol. 185, pp. 554–562.

    Article  Google Scholar 

  22. Bukhov, N.G., Dynamic Light Regulation of Photosynthesis (A Review), Fiziol. Rast. (Moscow), 2004, vol. 51, pp. 825–837 (Russ. J. Plant Physiol., Engl. Transl., pp. 742–753).

    Google Scholar 

  23. Satoh, K., Fluorescence Induction and Activity of Ferredoxin-NADP+ Reductase in Bryopsis Chloroplasts, Biochim. Biophys. Acta, 1981, vol. 638, pp. 327–331.

    Google Scholar 

  24. Scheibe, R., Redox-Modulation of Chloroplast Enzymes, Plant Physiol., 1991, vol. 96, pp. 1–3.

    Google Scholar 

  25. Knaff, D.R. and Hirasawa, M., Ferredoxin-Dependent Chloroplast Enzymes, Biochim. Biophys. Acta, 1991, vol. 1056, pp. 93–125.

    PubMed  Google Scholar 

  26. Bukhov, N.G., Makarova, V.M., and Krendeleva, T.E., Coordinated Changes in the Redox State of Photosystems I and II in Sunflower Leaves at Different Irradiances, Fiziol. Rast. (Moscow), 1998, vol. 45, pp. 645–652 (Russ. J. Plant Physiol., Engl. Transl., pp. 551–557).

    Google Scholar 

  27. Nikolaeva, M.K., Activation of Ferredoxin-NADP+ Oxidoreductase in Vicia faba Leaves Induced by a Short-Term Increase in Irradiance, Fiziol. Rast. (Moscow), 2001, vol. 48, pp. 698–704 (Russ. J. Plant Physiol., Engl. Transl., pp. 601–607).

    Google Scholar 

  28. Witt, Y.T., Coupling of Quanta, Electrons, Fields, Ions and Phosphorylation in the Functional Membrane of Photosynthesis; Results by Pulse Spectrophotometric Methods, Quart. Rev. Biophys., 1971, vol. 4, pp. 365–477.

    Google Scholar 

  29. Harbinson, J. and Hedley C.L., The Kinetics of P700+ Reduction in Leaves: A Novel In Situ Probe of Thylakoid Functioning, Plant Cell Environ., 1989, vol. 12, pp. 357–369.

    Google Scholar 

  30. Genty, B., Harbinson, J., and Baker, N., Relative Quantum Efficiencies of the Two Photosystems in Leaves in Photorespiratory and Non-Photorespiratory Conditions, Plant Physiol. Biochem., 1990, vol. 28, pp. 1–10.

    Google Scholar 

  31. Laisk, A. and Oja, V., Range of Photosynthetic Control of Postillumination P700+ Reduction Side in Sunflower Leaves, Photosynth. Res., 1994, vol. 39, pp. 39–50.

    Article  Google Scholar 

  32. Joliot, P. and Joliot, A., Cyclic Electron Transfer in Plant Leaf, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 10209–10214.

    Article  PubMed  Google Scholar 

  33. Bukhov, N.G., Carpentier, R., and Samson, G., Heterogeneity of Photosystem I Reaction Centers in Barley Leaves as Related to the Donation from Stromal Reductants, Photosynth. Res., 2001, vol. 70, pp. 273–279.

    Article  Google Scholar 

  34. Bukhov, N.G. and Egorova, E.A., Identification of Ferredoxin-Dependent Cyclic Electron Transport around Photosystem I by Means of the Kinetics of Dark P700+ Reduction, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 325–330 (Russ. J. Plant Physiol., Engl. Transl., pp. 283–287).

    Google Scholar 

  35. Egorova, E.A., Nikolaeva, M.K., and Bukhov, N.G., Origin of Multiphase Reduction of P700+ in Broad Bean Leaves after Irradiation with Far-Red Light, Fiziol. Rast. (Moscow), 2005, vol. 52, pp. 492–498 (Russ. J. Plant Physiol., Engl. Transl., pp. 434–440).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    M. K. Nikolaeva, N. G. Bukhov & E. A. Egorova

Authors
  1. M. K. Nikolaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. G. Bukhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Egorova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

From Fiziologiya Rastenii, Vol. 52, No. 4, 2005, pp. 485–491.

Original English Text Copyright © 2005 by Nikolaeva, Bukhov, Egorova.

The article was translated by the authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nikolaeva, M.K., Bukhov, N.G. & Egorova, E.A. Activities of Noncyclic and Alternative Pathways of Photosynthetic Electron Transport in Leaves of Broad Bean Plants Grown at Various Light Irradiances. Russ J Plant Physiol 52, 427–433 (2005). https://doi.org/10.1007/s11183-005-0063-0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11183-005-0063-0

Key words

Search

Navigation