Skip to main content
SpringerLink
Log in

Molecular profiling of silkworm biodiversity in India: An overview

  • Molecular Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Molecular tools opened a new vista to understand nature’s bio-diversity and its relevance and the same approach was availed of to build-up the foundation work on the bio-diversity of silkworm spp in India. It is well established that the heritage of usage of silk for dress materials in India, Russia, and China dates back to pre-medieval period and in spite of industrial development resulting to de-forestation, India still can claim as the owner of wide bio-diversity, espicially in northern India for silkworm spp. The molecular diversity was assessedamong Antheraea mylitta, A. assama, A. pernyi, A. proylei, A. roylei and Philcosomia cynthia wiht 11 ISSR and 8 non-random primers on agarose gel. Nei’s statistics as also Euclidean distance matrix was applied to find the genetic diversity between the six species, wherein the closest relationship between A. pernyi and A. proylei is established. With the help of POPGEN statistics, the average genetic heterozygosity appeared as 0.271 while Shanon’s index is 0.4312 and alleles with segregation ratios of 3:1, 2:1, 1:1, 9:1 (generated with ISSR primers) were identified which can be utilized for future molecular breeding program. Further, and attempt was made to isolate a number of bands generated with 3 ISSR and six non-random primers, specific for different species and 22 such markers have been characterized through sequencing which will be made available through international public domain database.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCE

  1. Wardle, T., Handbook of Indian Wild Silk, 1881.

  2. Watt, G., The Economic Products of India, 1883, vol. VI.

  3. Anonymous, Chinese Silk, China Silk Corporation, 1982.

  4. T.W. Rhys Davis (1903) Buddhist India Unwin London

    Google Scholar 

  5. Warmington, E.H., Commerce between the Roman Empire and India, Cambridge Univ. Press, 1928.

  6. R.K. Datta (1984) ArticleTitleImprovement of Silkworm Races, Bombyx mori L., in India Sericologia 24 393–415

    Google Scholar 

  7. Jolly, M.S., Sinha, B.R.P.D., and Sinha, S.S., Interspecific Hybridization in Antheraea (Lep., Saturnidae) with Special Reference to A. roylei Mr. and A. pernyi G.M., Proc. XII Int. Silk Congr., Barcelona, 1973.

  8. S. Okazaki H. Ishikawa H. Fujiwara (1995) ArticleTitleStructural Analysis of TRAS1, a Novel Family of Telomeric Repeat-Associated Retrotransposons in the Silkworm Bombyx mori, Mol. Cell. Biol. 15 4545–4552

    Google Scholar 

  9. H. Takahasi S. Okazaki H. Fujiwara (1997) ArticleTitleA New Family of Site-Specific Retrotransposons, SART1, Is Inserted into Telomeric Repeats of the Silkworm Bombyx mori, Nucleic Acids Res. 25 1578–1584

    Google Scholar 

  10. M.S. Jolly S.K. Sen (1974) Maqbool Ahsan Tasar Culture Central Silk Board India

    Google Scholar 

  11. J. Sambrook E.F. Fritsch T. Maniatis (1991) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Lab. Cold Spring Harbor, New York

    Google Scholar 

  12. S.N. Chatterjee T.P. Mohandas (2003) ArticleTitleIdentification of ISSR Markers Associated with Productivity Traits in Silkworm Bombyx mori L., Genome 46 438–447

    Google Scholar 

  13. M.R. Anderberg (1973) Cluster Analysis for Applications Academic New York

    Google Scholar 

  14. M. Nei (1973) ArticleTitleAnalysis of Gene Diversity in Subdivided Populations Proc. Natl. Acad. Sci. USA 70 3321–3323

    Google Scholar 

  15. M. Nei (1978) ArticleTitleEstimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals Genetics 89 583–590

    Google Scholar 

  16. S. Wright (1978) Variability within and among Natural Populations Univ. of Chicago Press Chicago

    Google Scholar 

  17. J.M. McDermott B.A. McDonald (1993) ArticleTitleGene Flow in Plant Patho Systems Annu. Rev. Phytopathol. 31 353–373

    Google Scholar 

  18. F. Arenal G. Platas J. Martin et al. (1999) ArticleTitleEvaluation of Different PCR-Based DNA Fingerprinting Techniques for Assessing the Genetic Variability of Isolates of the Fungus Epicoccum nigrum J. Appl. Microbiol. 87 898–906

    Google Scholar 

  19. S.R. Prasanth M. Parani B.P. Mohanty et al. (2002) ArticleTitleGenetic Diversity in Cultivars and Landraces of Oryza sativa subsp. indica as Revealed by AFLP Markers Genome 45 451–459

    Google Scholar 

  20. B. Bornet F. Goraguer G. Joly et al. (2002) ArticleTitleGenetic Diversity in European and Argentinean Cultivated Potatoes (Solanum tuberosum subsp. tuberosum) Detected by Inter-Simple Sequence Repeats (ISSRs) Genome 45 481–484

    Google Scholar 

  21. K.K. Nkongolo P. Michael W.S. Gratton (2002) ArticleTitleIdentification and Characterization of RAPD Markers Inferring Genetic Relationships among Pine Species Genome 45 51–58

    Google Scholar 

  22. T.Z. Ye R.-C. Yang F.C. Yeh (2002) ArticleTitlePopulation Structure of a Lodgepole Pine (Pinus contorta) and Jack Pine (P. banksiana) Complex as Revealed by Random Amplified Polymorphic DNA Genome 45 530–540

    Google Scholar 

  23. E. Zietkiewicz A. Rafalski D. Labuda (1994) ArticleTitleGenome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification Genomics 20 176–183 Occurrence Handle10.1006/geno.1994.1151 Occurrence Handle1:CAS:528:DyaK2cXmsVOhtbo%3D Occurrence Handle8020964

    Article  CAS  PubMed  Google Scholar 

  24. D.K. Reddy E.G. Abraham J. Nagaraju (1999) ArticleTitleMicrosatellites in the Silkworm, Bombyx mori: Abudance, Polymorphism, and Strain Characterization Genome 42 1057–1065

    Google Scholar 

  25. H. Akagi Y. Yokozeki A. Inagaki et al. (1996) ArticleTitleCodominant DNA Markers Closely Linked to the Rice Nuclear Restorer Gene, Rf-1, Identified with Inter-SSR Finger-printing. Genome 39 1205–1209 Occurrence Handle1:STN:280:ByiC3sjosFU%3D Occurrence Handle8983188

    CAS  PubMed  Google Scholar 

  26. T. Nagaoka Y. Ogihara (1997) ArticleTitleApplicability of Inter-Simple Sequence Repeat Polymorphism in Wheat for Use as DNA Markers in Comparison to RFLP and RAPD Markers Theor. Appl. Genet. 94 597–602

    Google Scholar 

  27. S.N. Chatterjee T.P. Mohandas T. Tanushree (2003) ArticleTitleMolecular Characterization of the Gene Pool of Exorista sorbillans (Diptera: Tachninidae), a Parasitoid of Silkworm, Bombyx mori, in India Eur. J. Entomol. 100 195–200

    Google Scholar 

  28. S.N. Chatterjee K. Vijayan G.C. Roy C.V. Nair (2004) ArticleTitleMolecular Characterization of the Genetic Variability in the Ecotypes of Antheraea mylitta, Drury, the Tropical Tasar Silkworm Russ. J. Genet. 40 IssueID2 152–159

    Google Scholar 

  29. Vijayan, K. and Chatterjee, S.N., ISSR Profiling of Indian Cultivars of Mulberry (Morus species) and Its Relevance to Breeding Programs, Euphytica, 2003, vol. 131.

  30. Vijayan, K., Nair, V.G., and Chatterjee, S.N., Molecular Characterization of Mulberry Genetic Resources Indigenous to India, Genet. Res. Crop Evol., 2003.

  31. S.N. Chowdhury (1970) Muga Culture Directorate of Sericulture and Weaving Assam (India) 15–76

    Google Scholar 

  32. W.J.B.A. Crotch (1956) ArticleTitleSilkmoths Rearer’s Handbook Ameture Entomologists’ Soc. 12 55–130

    Google Scholar 

  33. M.S. Jolly M.N. Narasimhanna S.K. Sen et al. (1971) ArticleTitleThe Pattern of Inheritance and Study of Morphology in Inter-specific Hybrid (Anthraea frithi, Mr. × Antheraea mylitta, D.) Ind. J. Seric. 10 42–49

    Google Scholar 

  34. G.S.C. Buso P.H.N. Rangel M.E. Ferreira (2001) ArticleTitleAnalysis of Random and Specific Sequences of Nuclear and Cytoplasmic DNA in Diploid and Tetraploid American Wild Rice Species (Oryza species) Genome 44 476–494

    Google Scholar 

  35. A.A.M. Dally (1990) ArticleTitleSecond Chloroplast DNA Diversity in Wild and Cultivated Species of Rice (Genus Oryza, Section oryza): Cladistic-Mutation and Genetic Distance Analysis Theor. Appl. Genet. 80 209–222

    Google Scholar 

  36. B. Demesure N. Sodzi R.J. Petit (1995) ArticleTitleA Set of Universal Primers for Amplification of Polymorphic Non-Coding Regions of Mitochondrial and Chloroplast DNA in Plants Mol. Ecol. 4 129–131

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Genetics, SeriBiotech Laboratory, Central Silk Board, Kodathi Campus, 560 035, Bangalore, Karnataka, India

    S. N. Chatterjee & T. Tanushree

Authors
  1. S. N. Chatterjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Tanushree
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

From Genetika, Vol. 40, No. 12, 2004, pp. 1618–1627.

Original English Text Copyright © 2004 by Chatterjee, Tanushree.

This article was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chatterjee, S.N., Tanushree, T. Molecular profiling of silkworm biodiversity in India: An overview. Russ J Genet 40, 1339–1347 (2004). https://doi.org/10.1007/s11177-005-0054-4

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11177-005-0054-4

Keywords

Search

Navigation