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Sequence-specific transport of oligonucleotides into human endothelial cells

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Abstract

This study is devoted to interactions of oligonucleotides containing specific DNA motifs with and transport into human endothelial cells. Those motifs are overrepresented in a pool of short DNA firmly bound to the surfaces of these cells. The data obtained by various methods suggest that the binding of oligonucleotides to the cell membrane and their accumulation in endothelial cells depend on both the presence of specific DNA motifs (CATGCAT, GATCCA, or TACGT) and their combination in the oligonucleotides under study. The oligonucleotides penetrate into endothelial cells via receptor-mediated endocytosis, which is confirmed by flow cytometry and fluorescence microscopy in the presence of potential inhibitors and competitors of intracellular transport.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8 prosp. Akad. Lavrent´eva, 630090, Novosibirsk, Russian Federation

    V. S. Chernonosova, Zh. K. Nazarkina, I. A. Zaporozhchenko & P. P. Laktionov

  2. E. N. Meshalkin State Research Institute of Circulation Pathology, 15 ul. Rechkunovskaya, 630055, Novosibirsk, Russian Federation

    V. S. Chernonosova, Zh. K. Nazarkina & P. P. Laktionov

Authors
  1. V. S. Chernonosova
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  2. Zh. K. Nazarkina
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  3. I. A. Zaporozhchenko
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  4. P. P. Laktionov
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Correspondence to V. S. Chernonosova.

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Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 6, pp. 1464–1469, June, 2015.

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Chernonosova, V.S., Nazarkina, Z.K., Zaporozhchenko, I.A. et al. Sequence-specific transport of oligonucleotides into human endothelial cells. Russ Chem Bull 64, 1464–1469 (2015). https://doi.org/10.1007/s11172-015-1033-0

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  • DOI: https://doi.org/10.1007/s11172-015-1033-0

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