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Nephrotoxicity assessments of benzo(a)pyrene during zebrafish embryogenesis

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Abstract

Benzo(a)pyrene is a chemical produced during the process of making fried, roasted, and smoked foods. It remains unclear whether benzo(a)pyrene affects the early development of human organs. In this study, we used the transgenic zebrafish line Tg(wt1b:GFP) as a model to assess benzo(a)pyrene-induced kidney malformation. By soaking zebrafish embryos in benzo(a)pyrene at various doses (2, 20, and 200 ppb), only a minor effect on the survival rate was detected (0 ppb: 97.8 ± 1.9 %; 2–200 ppb: 89.1 ± 5.8–91.5 ± 8.3 %). However, benzo(a)pyrene significantly affected the development of the kidney (malformation rates ranges from 50.0 ± 3.5 to 77.4 ± 5.3 %). Various abnormalities, such as unusual curving of pronephric tubes, swollen glomerulus, and incomplete development of pronephric ducts, were observed. This study provides a rapid and effective protocol for the evaluation of the notable effects of benzo(a)pyrene on embryonic kidney development.

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Acknowledgments

We are grateful to Dr. Englert (Fritz Lipmann Institute, Germany), who provided the Tg(wt1b:GFP) fish. This project was supported by the National Science Council, Republic of China.

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Authors and Affiliations

  1. Department of Chemistry, Tamkang University, No. 151 Ying-chuan Road, Tamsui District, New Taipei City 251, Taiwan

    Kang-Chieh Lo, Yu-Ju Ding & Yau-Hung Chen

  2. Department of Nephrology, Chang Gung Memorial Hospital, Keelung, Taiwan

    Chiao-Yin Sun

  3. School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan

    Jen-Ning Tsai

  4. Taipei Fuhsing Private School, Taipei, Taiwan

    Kai-Ping Chang, Yi-En Wen & Wan-Ling Chang

  5. Institute of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan

    Shin C. Chang

  6. Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei, Taiwan

    Ming-Fu Chang

  7. Division of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan

    Yun-Hsin Wang

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  1. Kang-Chieh Lo
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  2. Chiao-Yin Sun
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  8. Shin C. Chang
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  9. Ming-Fu Chang
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  10. Yun-Hsin Wang
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  11. Yau-Hung Chen
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Correspondence to Yau-Hung Chen.

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Lo, KC., Sun, CY., Ding, YJ. et al. Nephrotoxicity assessments of benzo(a)pyrene during zebrafish embryogenesis. Res Chem Intermed 40, 2177–2185 (2014). https://doi.org/10.1007/s11164-014-1595-8

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  • DOI: https://doi.org/10.1007/s11164-014-1595-8

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