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High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss


This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity (α-amylase, α-glucosidase, and lipase) and the antioxidant properties (DPPH, ABTS, FRAP, and β-carotene bleaching tests) of ethanol extracts of bulbs (BE) and aerial parts (APE) from Allium commutatum Guss. (known in Italy as “aglio delle isole”). The chemical profile revealed alliin as the main abundant compound with values of 31.5 and 38.8 mg/g extract for BE and APE, respectively. APE is rich also in quercetin (38.5 mg/g extract) and luteolin (31.8 mg/g extract). Bulbs extract exhibited the highest activity as inhibitor of enzymes linked to obesity. Except for DPPH test, APE showed the highest antioxidant potential with IC50 of 7.6 and 56.6 μg/mL in ABTS and β-carotene bleaching test after 60 min of incubation, respectively. In conclusion, the present investigation revealed A. commutatum bulbs and aerial parts as a promising source of inhibitors of enzyme linked to the obesity and of antioxidant compounds.

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Fig. 1



Aerial Parts Extract


2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)


Bulbs Extract


Butylated Hydroxytoluene


Diabetes Mellitus Type 2




Ferric Reducing Ability Power


Global Antioxidant Score


High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry

IC50 :

Half Maximal Inhibitory Concentration


Reactive Oxygen Species


Standard Deviation


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Correspondence to Rosa Tundis.

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Loizzo, M.R., Tundis, R., Sut, S. et al. High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss. Plant Foods Hum Nutr 75, 124–130 (2020).

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  • Garlic
  • Aerial parts
  • Bulbs
  • Carbohydrate-hydrolysing enzymes inhibition
  • Lipase inhibition
  • Antioxidant activity