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High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss

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Abstract

This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity (α-amylase, α-glucosidase, and lipase) and the antioxidant properties (DPPH, ABTS, FRAP, and β-carotene bleaching tests) of ethanol extracts of bulbs (BE) and aerial parts (APE) from Allium commutatum Guss. (known in Italy as “aglio delle isole”). The chemical profile revealed alliin as the main abundant compound with values of 31.5 and 38.8 mg/g extract for BE and APE, respectively. APE is rich also in quercetin (38.5 mg/g extract) and luteolin (31.8 mg/g extract). Bulbs extract exhibited the highest activity as inhibitor of enzymes linked to obesity. Except for DPPH test, APE showed the highest antioxidant potential with IC50 of 7.6 and 56.6 μg/mL in ABTS and β-carotene bleaching test after 60 min of incubation, respectively. In conclusion, the present investigation revealed A. commutatum bulbs and aerial parts as a promising source of inhibitors of enzyme linked to the obesity and of antioxidant compounds.

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Abbreviations

APE:

Aerial Parts Extract

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

BE:

Bulbs Extract

BHT:

Butylated Hydroxytoluene

DM2:

Diabetes Mellitus Type 2

DPPH:

2,2-Diphenyl-1Picrylhydrazyl

FRAP:

Ferric Reducing Ability Power

GAS:

Global Antioxidant Score

HPLC-ESI-MSn :

High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry

IC50 :

Half Maximal Inhibitory Concentration

ROS:

Reactive Oxygen Species

SD:

Standard Deviation

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Authors and Affiliations

  1. Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036, Rende (CS), Italy

    Monica R. Loizzo, Rosa Tundis, Mariarosaria Leporini & Tiziana Falco

  2. Dipartimento di Agronomia, Animali, Alimenti, Risorse naturali e Ambiente, University of Padova, Padova, Italy

    Stefania Sut

  3. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stefano Dall’Acqua

  4. Department of Earth and Marine Sciences, University of Palermo, Via Archirafi, 26, Palermo, Italy

    Vincenzo Ilardi

  5. Department of Agricultural Science, Mediterranean University of Reggio Calabria, 89123, Reggio Calabria, Italy

    Vincenzo Sicari

  6. Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Parco d’Orleans II, IT-90128, Palermo, Italy

    Maurizio Bruno

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  1. Monica R. Loizzo
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  2. Rosa Tundis
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Correspondence to Rosa Tundis.

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Loizzo, M.R., Tundis, R., Sut, S. et al. High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss. Plant Foods Hum Nutr 75, 124–130 (2020). https://doi.org/10.1007/s11130-019-00782-2

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