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Antithrombotic and Antioxidant Activity of Amaranth Hydrolysate Obtained by Activation of an Endogenous Protease

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Abstract

Ingestion of diets with antithrombotic and antioxidant components offer a convenient and effective way to prevent and reduce the incidence of cardiovascular diseases. The aim of the present work was to obtain an amaranth hydrolysate by the activation of an endogenous aspartic protease, to establish adequate experimental conditions, and to evaluate its antithrombotic and antioxidant activity in order to assess its potential application as an ingredient in functional foods. The results obtained not only confirmed the presence of an endogenous protease in the amaranth isolate, but also allowed us to select an adequate incubation conditions (pH 2, 40 °C, 16 h). The hydrolysate obtained (degree of hydrolysis 5.3 ± 0.4 %) showed potential antithrombotic activity (IC50 = 5.9 ± 0.1 mg soluble protein/mL) and had more antioxidant activity than the isolate, indicating that the activation of the protease released bioactive peptides from amaranth proteins. Decreasing the pH is a simple and cheap process and is another way to obtain potential functional ingredients with bioactive compounds.

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Abbreviations

DH%:

Degree of hydrolysis

IC50 :

Concentration of isolate or hydrolysate that inhibits 50 % of the thrombus formation or produces 50 % radical neutralization

HEP :

Hydrolysate prepared by activation of endogenous protease

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Acknowledgments

This work was supported by PIP - CONICET 11220110101109.

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Authors and Affiliations

  1. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Calle 47 y 116, 1900, La Plata, Argentina

    Ana Clara Sabbione, E. Nora Martínez, María Cristina Añón & Adriana A. Scilingo

  2. Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina

    Ana Clara Sabbione, Sabrina M. Ibañez, E. Nora Martínez, María Cristina Añón & Adriana A. Scilingo

  3. CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), CCT-La Plata, La Plata, Argentina

    Ana Clara Sabbione, E. Nora Martínez & María Cristina Añón

Authors
  1. Ana Clara Sabbione
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  2. Sabrina M. Ibañez
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  3. E. Nora Martínez
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  4. María Cristina Añón
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  5. Adriana A. Scilingo
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Corresponding author

Correspondence to Adriana A. Scilingo.

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Conflict of Interest

Authors, A.C. Sabbione, S.M. Ibañez, E.N. Martínez, M.C. Añón, and A. Scilingo, declare that they have no conflict of interest.

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Sabbione, A.C., Ibañez, S.M., Martínez, E.N. et al. Antithrombotic and Antioxidant Activity of Amaranth Hydrolysate Obtained by Activation of an Endogenous Protease. Plant Foods Hum Nutr 71, 174–182 (2016). https://doi.org/10.1007/s11130-016-0540-y

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  • DOI: https://doi.org/10.1007/s11130-016-0540-y

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