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Anti-hyperglycemic Effect of the Aqueous Extract of Banana Infructescence Stalks in Streptozotocin-induced Diabetic Rats

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Abstract

Water extract of banana (Musa sapientum) infructescence stalks has been used in folk medicine in the treatment of diabetes mellitus. This work aims at verifying the claimed effect and elucidating its possible mode of action. The extract was given in replacement of drinking water to diabetic rats, and its mechanism of action was studied by investigating its involvement in glucose transport in Caco-2 monolayers, and in rat jejuna using an in situ perfusion technique. Its effect on the Na+/K+ ATPase was studied by measuring the amount of inorganic phosphate liberated. The extract reduced significantly blood glucose levels in diabetic rats and glucose transport across rat jejuna and Caco-2 monolayers, and induced a 50 % decrease in their Na+/K+ ATPase activity. The extract did not induce any further decrease in jejunal glucose uptake in the simultaneous presence of phloridzin and phloretin, respective inhibitors of SGLT1 and GLUT2 transporters nor did it induce a change in the protein expression of SGLT1 and GLUT2. It was concluded that the extract acts by reducing the Na+/K+ ATPase activity of enterocytes and consequently the sodium gradient required for sugar transport by SGLT1, which leads to down-regulation of GLUT2 and contributes to the observed anti-hyperglycemic effect.

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Abbreviations

BISE:

Banana infructescence stalks extract

References

  1. Brownlee M (2001) Biochemistry and molecular cell biology of diabetic complications. Nature 414:813–820

    Article  CAS  Google Scholar 

  2. Nathan DM (1993) Long term complications of diabetes mellitus. New Engl J Med 328:1676–1685

    Article  CAS  Google Scholar 

  3. Omoruyi FO (2008) Jamaican bitter yam sapogenin: potential mechanisms of action in diabetes. Plant Foods Hum Nutr 63:135–140

    Article  Google Scholar 

  4. Büyükbalci A, El SN (2008) Determination of in vitro antidiabetic effects, antioxidant activities and phenol contents of some herbal teas. Plant Foods Hum Nutr 63:27–33

    Article  Google Scholar 

  5. Dixit Y, Kar A (2010) Protective role of three vegetable peels in alloxan induced diabetes mellitus in male mice. Plant Foods Hum Nutr 65:284–289

    Article  CAS  Google Scholar 

  6. Ojewole JA, Adewunmi C (2003) Hypoglycemic effect of methanolic extract of Musa paradisiaca (Musaceae) green fruits in normal and diabetic mice. Methods Find Exp Clin Pharmacol 25:453–456

    Article  CAS  Google Scholar 

  7. Gomathy R, Vijayalekshmi NR, Kurup PA (1990) Hypoglycemic action of the pectin present in the juice of the inflorescence stalk of plantain (Musa sapientum)—mechanism of action. J Biosci 15:297–303

    Article  CAS  Google Scholar 

  8. Bhaskar JJ, Salimath PV, Nandini CD (2011) Stimulation of glucose uptake by Musa sp. (cv. elakki bale) flower and pseudostem extracts in Ehrlich ascites tumor cells. J Sci Food Agric 91:1482–1487

    Article  CAS  Google Scholar 

  9. Dikshit P, Shackle K, Tyagi MK, Garg P, Gambhir JK, Shukla R (2012) Antidiabetic and antihyperlipidemic effect of stem of Musa sapientum Linn. in streptozotocin-induced diabetic rats. J Diabetes. doi:10.1111/j.1753-0407.2012.00198.x

  10. Crane RK (1997) The gradient hypothesis and other models of carrier-mediated transport. Rev Physiol Biochem Pharmacol 78:99–159

    Article  Google Scholar 

  11. Chen W, Tang F, Horie K, Borchardt R (2002) Caco-2 cell monolayers as a model for studies of drug transport across human intestinal epithelium. In: Lehr CM (ed) Cell Culture Models of Biological Barriers. In vitro Test Systems for Drug Absorption and Delivery, London and New York, CRC Press, 143–163

  12. Esmann E (1988) ATPase and phosphatase activity of Na+, K+ -ATPase: molar and specific activity, protein determination. In: Fleischer S, Fleischer B (eds) Methods in enzymology. Vol. 156. Biomembranes part P—ARP-driven pumps and related transport: the Na, K-pump. Academic Press, London, pp 105–115

    Chapter  Google Scholar 

  13. Taussky HH, Shorr E (1953) Microcalorimetric method for determination of inorganic phosphorous. J Biol Chem 202:675–685

    CAS  Google Scholar 

  14. Jakus V, Rietbrock N (2004) Advanced glycation end-products and the progress of diabetic vascular complications. Physiol Res 53:131–142

    CAS  Google Scholar 

  15. Sambuy Y, De Angelis I, Ranald G, Scarino ML, Stammati A, Zucco F (2005) The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol 21:1–26

    Article  CAS  Google Scholar 

  16. Crane R (1961) An effect of alloxan-diabetes on the active transport of sugars by rat small intestine, in vitro. Biochem Biophys Res Commun 4:436–440

    Article  CAS  Google Scholar 

  17. Gorboulev V, Schürmann A, Vallon V, Kipp H, Jaschke A, Klessen D et al (2012) Na+-D-glucose cotransporter SGLT1 is pivotal for intestinal glucose absorption and glucose-dependent incretin secretion. Diabetes 61:187–196

    Google Scholar 

  18. Gnanaprakasam MS, Srivastava LM (1978) Effect of starvation, alloxan diabetes and adrenalectomy on Na+ K+-ATPase of the mucosa of the small intestine of rat. Biochem Exp Biol 14:257–262

    CAS  Google Scholar 

  19. Debnam ES, Karasov WH, Thompson CS (1988) Nutrient uptake by rat enterocytes during diabetes mellitus; evidence for an increased sodium electrochemical gradient. J Physiol 397:503–512

    CAS  Google Scholar 

  20. Lopina D (2001) Interaction of Na, K_ATPase catalytic subunit with cellular proteins and other endogenous regulators. Biochem (Moscow) 66:1122–1131

    Article  CAS  Google Scholar 

  21. Hediger MA, Coady MJ, Ikeda TS, Wright EM (1987) Expression cloning and cDNA sequencing of the Na+/glucose co-transporter. Nature 330:379–381

    Article  CAS  Google Scholar 

  22. Lee WS, Kanai Y, Wells RG, Hediger MA (1994) The high affinity Na+/glucose cotransporter. Re-evaluation of function and distribution of expression. J Biol Chem 269:12032–12039

    CAS  Google Scholar 

  23. Takata K, Kasahara T, Kasahara M, Ezaki O, Hirano H (1992) Immunohistochemical localization of Na+-dependent glucose transporter in rat jejunum. Cell Tissue Res 26:3–9

    Article  Google Scholar 

  24. Kellet GL, Brot-Laroche E (2005) Apical GLUT2, A major pathway of intestinal sugar absorption. Diabetes 54:3056–3062

    Article  Google Scholar 

  25. Kellett GL, Helliwell PA (2000) The diffusive component of intestinal glucose absorption is mediated by the glucose induced recruitment of GLUT2 to the brush-border membrane. Biochem J 350(Pt 1):155–162

    Article  CAS  Google Scholar 

  26. Knapp FF, Nicholas HJ (1969) The sterols and triterpenes of banana pulp. J Food Sci 34:584–586

    Article  CAS  Google Scholar 

  27. Waalkes TP, Sjoerdsma A, Creveling CR, Weissbach H, Udenfriend S (1958) Serotonin, norepinephrine and related compounds in banana. Science 127:646–650

    Article  CAS  Google Scholar 

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Acknowledgement

This work was supported by a grant from the University Research Board.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts & Sciences, American University of Beirut, Bliss str, Beirut, Lebanon

    Hwaida Jaber, Elias Baydoun, Ola EL-Zein & Sawsan Ibrahim Kreydiyyeh

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  1. Hwaida Jaber
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  2. Elias Baydoun
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  3. Ola EL-Zein
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  4. Sawsan Ibrahim Kreydiyyeh
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Correspondence to Sawsan Ibrahim Kreydiyyeh.

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Jaber, H., Baydoun, E., EL-Zein, O. et al. Anti-hyperglycemic Effect of the Aqueous Extract of Banana Infructescence Stalks in Streptozotocin-induced Diabetic Rats. Plant Foods Hum Nutr 68, 83–89 (2013). https://doi.org/10.1007/s11130-013-0341-5

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