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Protective Role of Three Vegetable Peels in Alloxan Induced Diabetes Mellitus in Male Mice

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Abstract

The hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. In a preliminary study, effects of ethanolic extracts of Cucurbita pepo, Cucumis sativus and Praecitrullus fistulosus peels were studied at 250 and 500 mg kg−1 d−1 for 15 days in the alterations in serum glucose and in hepatic lipid peroxidation (LPO) in male mice. In the pilot experiment, the effective and safe concentration of each peel was administered (p.o.) for 10 consecutive days and then on 11th and 12th days alloxan was administered along with peel extracts. The treatment was continued up to 15th day. At the end, alterations in serum glucose, insulin, triiodothyronine, thyroxine, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, very low density lipoprotein, hepatic lipid peroxidation, superoxide dismutase and catalase were studied. All the three peel extracts nearly reversed most of these changes induced by alloxan suggesting their possible role in ameliorating diabetes mellitus and related changes in serum lipids. However, Cucurbita pepo peel was found to be the most effective. Total polyphenols, flavonoids and ascorbic acid contents of the test peels were also estimated, which appear to be associated with the observed antidiabetic and antioxidative potentials.

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Abbreviations

LPO:

Lipid peroxidation—oxidative degradation of lipids

SOD:

Superoxide dismutase—an endogenous antioxidant

CAT:

Catalase—an endogenous antioxidant

TBA:

Thiobarbituric acid—a chemical that reacts with malondialdehyde

TCA:

Tricarboxylic acid—a chemical that precipitates protein

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Acknowledgements

Financial support from the University Grant Commission, N. Delhi, India to Ms. Yamini Dixit is gratefully acknowledged. Some help from Dr. S. Panda and Dr. H. S. Parmar is also appreciated.

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  1. Endocrine Research Unit, School of Life Sciences,Takshashila Campus, Devi Ahilya Vishwavidhyalaya, Khandwa Road, Indore, MP, India, 452017

    Yamini Dixit & Anand Kar

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  1. Yamini Dixit
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  2. Anand Kar
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Correspondence to Yamini Dixit.

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Dixit, Y., Kar, A. Protective Role of Three Vegetable Peels in Alloxan Induced Diabetes Mellitus in Male Mice. Plant Foods Hum Nutr 65, 284–289 (2010). https://doi.org/10.1007/s11130-010-0175-3

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