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Determination of the Antioxidant Capacity of Culinary Herbs Subjected to Various Cooking and Storage Processes Using the ABTS*+ Radical Cation Assay

Abstract

Culinary herbs have the potential to be a significant source of antioxidants in the diet. However, many culinary herbs are cooked or undergo some other form of processing before they are consumed as part of a meal and such factors may affect their significance as a source of dietary antioxidants. Thus, the impact of cooking (simmering, microwaving, stewing, stir frying and grilling) and storage (vinegar maceration, cold maceration and freezing) on the antioxidant capacity of common culinary herbs was investigated. Extracts of cinnamon, cloves, fennel, ginger, lavender, parsley, rose, rosemary, sage and thyme were prepared pre and post cooking or storage and their antioxidant capacities determined using the Trolox equivalent antioxidant capacity assay (TEAC). Simmering, soup making and stewing significantly increased antioxidant capacity, whilst grilling and stir frying decreased it. Both freezing herbs at −20 °C and cold maceration had preservative effects on antioxidant capacity. Herbs in cold vinegar macerations for 1 week showed a decrease in antioxidant capacity compared to the control extracts. These results indicate that the potential of culinary herbs to be significant contributors to dietary antioxidant intake is significantly affected by both cooking and storage.

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Abbreviations

ABTS:

2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

DPPH:

diphenylpicryl-hydrazyl

FRAP:

ferric reducing/antioxidant power

TEAC:

Trolox equivalent antioxidant capacity

References

  1. 1.

    Southgate DAT (2000) Vegetables, fruits, fungi and their products. In: Garrow JS, James WPT, Ralph A (eds) Human Nutrition and Dietetics, 10th edn. Edinburgh/London/New York/Philadelphia/St Louis/Sydney/Toronto: Churchill Livingstone UK, pp. 393–394.

  2. 2.

    Crowell PL (1999) Prevention therapy of cancer by dietary monoterpenes. J Nutr 129:775s–778s

  3. 3.

    Mantle D, Edded F, Pickening AT (2000) Comparison of relative antioxidant activities of British medicinal plant species in vitro. J of Ethnopharmacol 72:47–51

  4. 4.

    Zheng W, Wang SY (2001) Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 49:5165–5170

  5. 5.

    Dragland S, Senoo H, Wake K, Holte K, Bolomhoff R (2003) Several culinary and medicinal herbs are important sources of dietary antioxidants. J Nutr 133:1286–1290

  6. 6.

    Khan MTH, Ather A, Thompson KD, Gambari R (2005) Extracts and molecules from medicinal plants against herpes simplex viruses. Antiviral Res 67:107–119

  7. 7.

    Kris-Etherton P, Hecker K, Bonanome A, Coval S, Binkoski A, Hilpert K, Griel A, Etherton T (2002) Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113:71s–88s

  8. 8.

    Craig WJ (1999) Health-promoting properties of common herbs. Am J Clin Nutr 70:491S–499S

  9. 9.

    Tapsell LC, Hemphill I, Cobiac L, Sullivan DR, Fenech M, Patch CS, Roodenrys S, Keogh JB, Clifton PM, Williams PG, Fazio VA, Inge KA (2006) Health benefits of herbs and spices: the past, the present, the future. Med J Aust 185(4 Suppl):S1–S24

  10. 10.

    Halvorsen BL, Holte K, Myhrstad M, Barikmo I, Hvattum E, Remberg SF, Wold AB, Haffner K, Baugerod H, Anderson L, Moskaug J, Jacobs DR, Blomhoff JR (2002) A systematic screening of total antioxidants in dietary plants. J Nutr 132:461–471

  11. 11.

    Halvorensen BL, Carlsen MH, Phillipis KM, Bøhn SK, Jacobs DR, Blomhoff JR (2006) Content of redox-active compounds (i.e., antioxidants) in foods consumed in the United States. Am J Clin Nutr 84:95–135

  12. 12.

    Ching S, Cheung C-M, Szeto Y-T, Benzie IFF (2007) Antioxidant protection of edible oils. Plant Food Hum Nutr 62:39–42

  13. 13.

    Prior R, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidants capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302

  14. 14.

    Re R, Pellegrini N, Proteggent A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant capacity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 2:6:1231–1237

  15. 15.

    Lemanska K, Szymusiak H, Tyrakowska B, Zielinski R, Soffers A, Rietijiens I (2001) The influence of pH on antioxidant properties and the mechanisms of antioxidants action of hydroxyflavones. Free Radic Biol Med 3:869–881

  16. 16.

    Crawley H (1988) Food portion size, 2nd edn. London: HMSO. The Stationary Office

  17. 17.

    Food Standards Agency (2002) McCance and Widdowson’s The Composition of Foods, 6th edn. Royal Society of Chemistry, Cambridge

  18. 18.

    Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M (2005) Plant genotypes affects total antioxidant capacity and phenolic contents in fruit. Nutrition 21:207–213

  19. 19.

    Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, Brighenti F (2003) Total antioxidant capacity of plant foods, beverages and oils consumed in Italy by three different in vitro assays. J Nutr 133:2812–2819

  20. 20.

    Wren RC, Williamson E (2003) Potter’s Herbal Cyclopaedia, Rev Ed edn. The CW Daniel Company Ltd, London

  21. 21.

    Hinneburg I, Dorman DHJ, Hiltunen R (2006) Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem 97:122–129

  22. 22.

    Benzie IFF, Strain JJ (1999) Ferric reducing antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 299:15–27

  23. 23.

    Choi Y, Lee SM, Chun J, Lee HB, Lee J (2006) Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99:381–387

  24. 24.

    Kim S, Jeong S, Park W, Nam KC, Ahn DU, Lee S (2006) Effect of heating conditions of grape seeds on the antioxidant activity of grape seed extracts. Food Chem 97:472–479

  25. 25.

    Tubaro F, Micossi E, Ursini E (1996) The antioxidant capacity of complex mixtures by kinetic analysis of crocin bleaching inhibition. J Am Oil Chem Soc 73:173–179

  26. 26.

    Hunter KJ, Fletcher JM (2002) The antioxidant capacity and composition of fresh, frozen, jarred and canned vegetables. Innov Food Sci Emerg 3:399–406

  27. 27.

    Manach C, Scalbert A, Morand C, Remesy C, Jimenez L (2003) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79:727–747

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Correspondence to Elizabeth I. Opara.

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Chohan, M., Forster-Wilkins, G. & Opara, E.I. Determination of the Antioxidant Capacity of Culinary Herbs Subjected to Various Cooking and Storage Processes Using the ABTS*+ Radical Cation Assay. Plant Foods Hum Nutr 63, 47–52 (2008) doi:10.1007/s11130-007-0068-2

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Keywords

  • ABTS*+/Trolox assay
  • Antioxidant capacity
  • Culinary herbs
  • Polyphenols