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Effect of cationic antiseptics on fluorescent characteristics and electron transfer in cyanobacterial photosystem I complexes

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Abstract

In this study, the effects of cationic antiseptics such as chlorhexidine, picloxidine, miramistin, and octenidine at concentrations up to 150 µM on fluorescence spectra and its lifetimes, as well as on light-induced electron transfer in protein-pigment complexes of photosystem I (PSI) isolated from cyanobacterium Synechocystis sp. PCC 6803 have been studied. In doing so, octenidine turned out to be the most “effective” in terms of its influence on the spectral and functional characteristics of PSI complexes. It has been shown that the rate of energy migration from short-wavelength forms of light-harvesting chlorophyll to long-wavelength ones slows down upon addition of octenidine to the PSI suspension. After photo-separation of charges between the primary electron donor P700 and the terminal iron-sulfur center(s) FA/FB, the rate of forward electron transfer from (FA/FB)− to the external medium slows down while the rate of charge recombination between reduced FA/FB− and photooxidized P700+ increases. The paper considers the possible causes of the observed action of the antiseptic.

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Funding

Funding was provided by Science Project of the State Order of the Government of Russian Federation (121032500058-7).

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Authors and Affiliations

  1. Biophysical Department, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskye Gory 1, Build. 12, Moscow, Russia, 119234

    Vladimir Z. Paschenko, Eugene P. Lukashev, Daniil A. Gvozdev & Peter P. Knox

  2. A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskye Gory 1, Build. 40, Moscow, Russia, 119992

    Mahir D. Mamedov

Authors
  1. Vladimir Z. Paschenko
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  2. Eugene P. Lukashev
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  3. Mahir D. Mamedov
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  4. Daniil A. Gvozdev
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  5. Peter P. Knox
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Contributions

Conceptualization: VZP, PPK. Methodology: EPL, MDM. Formal analysis and investigation: EPL, MDM, DAG. Writing—original draft preparation: MDM, PPK. Writing—review and editing: VZP, EPL, MDM, DAG, PPK. Supervision: VZP.

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Correspondence to Daniil A. Gvozdev.

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Paschenko, V.Z., Lukashev, E.P., Mamedov, M.D. et al. Effect of cationic antiseptics on fluorescent characteristics and electron transfer in cyanobacterial photosystem I complexes. Photosynth Res 159, 241–251 (2024). https://doi.org/10.1007/s11120-023-01039-5

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  • DOI: https://doi.org/10.1007/s11120-023-01039-5

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