Abstract
The chromophorylated PBLcm domain of the ApcE linker protein in the cyanobacterial phycobilisome (PBS) serves as a bottleneck for Förster resonance energy transfer (FRET) from the PBS to the antennal chlorophyll of photosystem II (PS II) and as a redirection point for energy distribution to the orange protein ketocarotenoid (OCP), which is excitonically coupled to the PBLcm chromophore in the process of non-photochemical quenching (NPQ) under high light conditions. The involvement of PBLcm in the quenching process was first directly demonstrated by measuring steady-state fluorescence spectra of cyanobacterial cells at different stages of NPQ development. The time required to transfer energy from the PBLcm to the OCP is several times shorter than the time it takes to transfer energy from the PBLcm to the PS II, ensuring quenching efficiency. The data obtained provide an explanation for the different rates of PBS quenching in vivo and in vitro according to the half ratio of OCP/PBS in the cyanobacterial cell, which is tens of times lower than that realized for an effective NPQ process in solution.
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Abbreviations
- APC:
-
Allophycocyanin
- FRP:
-
Fluorescence recovery protein
- FRET:
-
Förster resonance energy transfer
- hECN:
-
3’-Hydroxyechinenone
- NPQ:
-
Non-photochemical quenching
- OCP:
-
Orange carotenoid protein
- OCPO :
-
Dark-stable orange form of OCP
- OCPR :
-
Photoactive red form of OCP
- PBS(s):
-
Phycobilisome(s)
- PE:
-
C-phycoerythrin
- PS II:
-
Photosystem II
- RCP:
-
Red carotenoid protein
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The research was carried out within the state assignment of the Ministry of Science and Higher Education of the Russian Federation (Theme No 122042700044-6).
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PMK and INS designed the project. INS performed the fluorescence spectra measurements and wrote the manuscript. PMK performed the energy transfer calculations and evaluated the results.
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Stadnichuk, I.N., Krasilnikov, P.M. Relationship between non-photochemical quenching efficiency and the energy transfer rate from phycobilisomes to photosystem II. Photosynth Res 159, 177–189 (2024). https://doi.org/10.1007/s11120-023-01031-z
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DOI: https://doi.org/10.1007/s11120-023-01031-z