Abstract
The LH1 complexes were isolated from the purple photosynthetic bacterium Rhodospirillum rubrum strain S1. They were initially solubilized using LDAO and then purified in the presence of Triton X-100. The purified complexes were then either used directly or following an exchange into LDAO. Stark spectroscopy was applied to probe the electrostatic field around the bacteriochlorophyll a (BChl a) and carotenoid binding sites in the LH1 complexes surrounded by these two different surfactant molecules. Polarizabilty change (\(\Updelta\varvec{\upalpha}\)) and dipole moment change (\(\Updelta\varvec{\upmu}\)) upon photoexcitation were determined for the BChl a Qy band. Both of these parameters show smaller values in the presence of LDAO than in Triton X-100. This indicates that polar detergent molecules, like LDAO, affect the electrostatic environment around BChl a, and modify the nonlinear optical parameters (\(\Updelta\varvec{\upalpha}\) and \(\Updelta\varvec{\upmu}\) values). The electrostatic field around the BChl a binding site, which is generated by the presence of LDAO, was determined to be |E L | = ∼3.9 × 105 [V/cm]. Interestingly, this kind of electrostatic effect was not observed for the carotenoid-binding site. The present study demonstrates a unique electrostatic interaction between the polar detergent molecules surrounding the LH1 complex and the Qy absorption band of BChl a that is bound to the LH1 complex.
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Abbreviations
- BChl a :
-
Bacteriochlorophyll a
- LDAO:
-
N,N-lauryl dimethyl aminooxide
- LH1:
-
Light-harvesting 1 complex
- RC:
-
Reaction center
- Rs. rubrum S1:
-
Rhodospirillum rubrum strain S1
References
Akahane J, Rondonuwu FS, Fiedor L, Watanabe Y, Koyama Y (2004) Dependence of singlet-energy transfer on the conjugation length of carotenoids reconstituted into the LH1 complex from Rhodospirillum rubrum G9. Chem Phys Lett 393:184–191
Bahatyrova S, Frese RN, van der Werf KO, Otto C, Hunter CN, Olsen JD (2004) Flexibility and size heterogeneity of the LH1 light harvesting complex revealed by atomic force microscopy: functional significance for bacterial photosynthesis. Biol Chem 279:21327–21333
Benning C (1998) Membrane lipids in anoxygenic photosynthetic bacteria. In: Siegenthaler P-A, Murata N (eds) Lipids in photosynthesis: structure, function, and genetics, vol 6. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 83–101
Cogdell RJ, Crofts AR (1978) Analysis of the pigment content of an antenna pigment-protein complex from three strains of Rhodopseudomonas sphaeroides. Biochim Biophys Acta 502:409–416
Cohen-bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68
Fiedor L, Akahane J, Koyama Y (2004) Carotenoid-induced cooperative formation of bacterial photosynthetic LH1 complex. Biochemistry 43:16487–16496
Fotiadis D, Qian P, Philippsen A, Bullough PA, Engel A, Hunter CN (2004) Structural analysis of the reaction center light-harvesting complex I photosynthetic core complex of Rhodospirillum rubrum using atomic force microscopy. J Biol Chem 279:2063–2068
Gradinaru CC, Kennis JTM, Papagiannakis E, van Stokkum IHM, Cogdell RJ, Fleming GR, Niederman RA, van Grondelle R (2001) An unusual pathway of excitation energy deactivation in carotenoids: singlet-to-triplet conversion on an ultrafast timescale in a photosynthetic antenna. Proc Natl Acad Sci USA 98:2364–2369
Jamieson SJ, Wang P, Qian P, Kirkland JY, Conroy MJ, Hunter CN, Bullough PA (2002) Projection structure of the photosynthetic reaction centre-antenna complex of Rhodospirillum rubrum at 8.5 Å resolution. EMBO J 21:3927–3935
Karrasch S, Bulloughl PA, Ghosh R (1995) The 8.5 Å projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. EMBO J 14:631–638
Ketelaars M, Hofmann C, Köhler J, Howard TD, Cogdell RJ, Schmidt J, Aartsma TJ (2002) Spectroscopy on individual light-harvesting 1 complexes of Rhodopseudomonas acidophila. Biophys J 83:1701–1715
Kwa LG, Wegmann D, Wanner G, Braun P (2007) Mutation of a single residue, glutamate β10, alters protein-lipid interactions of light harvesting complex II. Presentation at PS2007 Satellite Meeting of Light-Harvesting Systems Workshop, 19th–22nd July in Drymen, Scotland, UK
Leupold D, Voigt B, Beenken W, Stiel H (2000) Pigment-protein architecture in the light-harvesting antenna complexes of purple bacteria: does the crystal structure reflect the native pigment-protein arrangement? FEBS Lett 480:73–78
Liptay W (1974) Dipole moments and polarizabilities of molecules in excited electronic states. In: Lin EC (ed) Excited states, vol 1. Academic Press, New York, USA, pp 129–229
Nagae H, Kuki M, Cogdell RJ, Koyama Y (1994) Shifts of the 1A −g → 1B +u electronic absorption of carotenoids in nonpolar and polar solvents. J Chem Phys 101:6750–6765
Picorel R, Bélanger G, Gingras G (1983) Antenna holochrome B880 of Rhodospirillum rubrum S1: pigment, phospholipid, and polypeptide composition? Biochemistry 22:2491–2497
Roszak AW, Howard TD, Southall J, Gardiner AT, Law CJ, Isaacs NW, Cogdell RJ (2003) Crystal structure of the RC-LH1 complex from Rhodopseudomonas palustris. Science 302:1969–1972
Yanagi K, Hashimoto H, Gardiner AT, Cogdell RJ (2004) Stark spectroscopy on the LH2 complex from Rhodobacter sphaeroides strain G1C: frequency and temperature dependence. J Phys Chem B 108:10334–10339
Yanagi K, Shimizu M, Hashimoto H, Gardiner AT, Roszak AW, Cogdell RJ (2005) Local electrostatic field induced by the carotenoid bound to the reaction center of the purple photosynthetic bacterium Rhodobacter sphaeroides. J Phys Chem B 109:992–998
Acknowledgements
This work was supported by the Grant-in-aid from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (Grants No. 17204026, No. 17654083). Hideki Hashimoto, Ritsuko Fujii, Mamoru Nango, Alastair T. Gardiner and Richard J. Cogdell thank for the financial support from SICP/JST. Hideki Hashimoto also acknowledges for the support from PRESTO/JST. Richard J. Cogdell and Alastair T. Gardiner thank for the financial support from BBSRC.
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Nakagawa, K., Suzuki, S., Fujii, R. et al. Electrostatic effect of surfactant molecules on bacteriochlorophyll a and carotenoid binding sites in the LH1 complex isolated from Rhodospirillum rubrum S1 probed by Stark spectroscopy. Photosynth Res 95, 345–351 (2008). https://doi.org/10.1007/s11120-007-9257-y
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DOI: https://doi.org/10.1007/s11120-007-9257-y