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Determination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides, and Phaeospirillum molischianum by Extraction of Bacteriochlorophyll a

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Abstract

The molar extinction coefficients of light-harvesting complex 2 (LH2) have been ambiguous in spite of its fame and wide utilization. Herein we determine the molar extinction coefficients of the LH2 proteins derived from the three purple photosynthetic bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides and Phaeospirillum molischianum at 298 K by direct extraction of bacteriochlorophyll (BChl) a from the lyophilized proteins, followed by estimation of BChl a amounts from their electronic absorption spectra.

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Acknowledgments

We thank Dr. Jiro Harada, Kurume University, for providing purple photosynthetic bacteria, and Mrs. Kanji Miyagi and Ryota Matsui, Kinki University, for their experimental assistance. This work was partially supported by a Grant-in-Aid for Scientific Research on Innovative Areas “Artificial Photosynthesis (AnApple)” (No. 15H00887) from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Engineering, Kinki University, Higashi-Osaka, Osaka, 577-8502, Japan

    Yoshitaka Saga & Keiya Hirota

  2. PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012, Japan

    Yoshitaka Saga

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  1. Yoshitaka Saga
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  2. Keiya Hirota
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Correspondence to Yoshitaka Saga.

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Saga, Y., Hirota, K. Determination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides, and Phaeospirillum molischianum by Extraction of Bacteriochlorophyll a. ANAL. SCI. 32, 801–804 (2016). https://doi.org/10.2116/analsci.32.801

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