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The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II

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Abstract

The PsbP-like protein of the cyanobacterium Synechocystis sp. PCC 6803 is a peripheral component of Photosystem II, located at the lumenal side of the thylakoid membrane. Removal of this protein leads to decreased competitive potential of a PsbP-like deletion mutant when grown in a mixture with wild-type cells. Flash-induced oxygen evolution traces of the mutant show a higher probability of misses, correlated with increased amplitudes of the S-states decay in the dark. Thermoluminescence emission traces demonstrate a changed charge recombination pattern in the mutant, the S3Q −B couple becoming the major species instead of the S2Q −B . Our data suggest a possible role of the PsbP-like protein in stabilisation of the charge separation in Photosystem II of cyanobacteria through interaction with the Mn cluster.

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Abbreviations

Chl:

Chlorophyll

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

PSII:

Photosystem II

Q A or B :

Quinone electron acceptor molecules in the reaction centre of Photosystem II (primary and secondary electron-accepting quinone respectively)

S 0−4 :

Oxidative states of the Photosystem II Mn cluster

YD :

Redox active tyrosine 160 on the D2 polypeptide of Photosystem II

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Acknowledgements

This work was financially supported by grants from the Swedish research council and Carl Tryggers foundation, Sweden. We thank Prof. Julian Eaton-Rye and Prof. Himadri Pakrasi for the generous gift of their PsbP-like protein mutant strains, and Prof. Johannes Messinger for providing the software used to calculate the probability of misses and double hits in flash-induced oxygen evolution measurements. We also thank Dr. Prafullachandra V. Sane for the introduction into the thermoluminescence world, Prof. Gunnar Öquist for providing the thermoluminescence apparatus, Dr. Andrei Borodich for mathematics support, and Dr. Yasuo Ishikawa for fruitful discussions which led to the initiation of this project.

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Authors and Affiliations

  1. Department of Chemistry, Umeå University, 901 87, Umeå, Sweden

    Dmitry Sveshnikov, Christiane Funk & Wolfgang P. Schröder

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  1. Dmitry Sveshnikov
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  2. Christiane Funk
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  3. Wolfgang P. Schröder
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Correspondence to Christiane Funk.

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Christiane Funk, Wolfgang P. Schröder contributed equally to this work.

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Sveshnikov, D., Funk, C. & Schröder, W.P. The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II. Photosynth Res 93, 101–109 (2007). https://doi.org/10.1007/s11120-007-9171-3

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  • DOI: https://doi.org/10.1007/s11120-007-9171-3

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